ABSTRACT
Purpose:
XinJiaCongRongTuSiZiWan (XJCRTSZW) is a traditional Chinese medicine compound for invigorating the kidney, nourishing blood, and promoting blood circulation. This study aimed to explore the effect of XJCRTSZW on triptolide (TP)-induced oxidative stress injury.
Methods:
Adult female Sprague-Dawley rats and human ovarian granulosa cell lines were treated with TP and XJCRTSZW. Hematoxylin and eosin staining, enzyme-linked immunosorbent assay, flow cytometry, CCK-8, JC-1 staining, transmission electron microscopy, reverse transcription-quantitative polymerase chain reaction, and Western blotting were performed in this study.
Results:
XJCRTSZW treatment observably ameliorated the TP-induced pathological symptoms. Furthermore, XJCRTSZW treatment observably enhanced the TP-induced reduction of estradiol, anti-Mullerian hormone, progesterone, superoxide dismutase, ATP content, mitochondrial membrane potential, p62, and Hsp60 mRNA, and protein levels in vivo and in vitro (p < 0.05). However, TP-induced elevation of follicle stimulating hormone and luteinizing hormone concentrations, malondialdehyde levels, reactive oxygen species levels, apoptosis rate, mitophagy, and the mRNA and protein expressions of LC3-II/LC3-I, PTEN-induced kinase 1 (PINK1), and Parkin were decreased (p < 0.05). In addition, XJCRTSZW treatment markedly increased cell viability in vitro (p < 0.05).
Conclusions:
XJCRTSZW protects TP-induced rats from oxidative stress injury via the mitophagy-mediated PINK1/Parkin pathway.
Key words:
Medicine, Chinese Traditional; Reproductive Medicine; Oxidative Stress; Mitophagy
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XJCRTSZW: XinJiaCongRongTuSiZiWan; TP: triptolide; EV: estradiol valerate; E2: estradiol; AMH: anti-Mullerian hormone; FSH: follicle stimulating hormone; LH: luteinizing hormone; *p < 0.05 compared to the control group; #p < 0.05 compared to the TP group; &p < 0.05 compared to the TP + XJCRTSZW-High group. Source: Elaborated by the authors.
XJCRTSZW: XinJiaCongRongTuSiZiWan; TP: triptolide; EV: estradiol valerate; SOD: superoxide dismutase; MDA: malondialdehyde; *p < 0.05 compared to the control group; #p < 0.05 compared to the TP group; &p < 0.05 compared to the TP + XJCRTSZW-High group. Source: Elaborated by the authors.
XJCRTSZW: XinJiaCongRongTuSiZiWan; TP: triptolide; EV: estradiol valerate; PINK1: PTEN-induced kinase 1; MMP: mitochondrial membrane potential; *p < 0.05 compared to the control group, #p < 0.05 compared to the TP group, &p < 0.05 compared to the TP + XJCRTSZW-High group. Source: Elaborated by the authors.
XJCRTSZW: XinJiaCongRongTuSiZiWan; TP: triptolide; EV: estradiol valerate; PINK1: PTEN-induced kinase 1; E2: estradiol; AMH: anti-Mullerian hormone; P: progesterone; *p < 0.05 compared to the control group, #p < 0.05 compared to the TP group, &p < 0.05 compared to the TP + XJCRTSZW-High group. Source: Elaborated by the authors.
XJCRTSZW: XinJiaCongRongTuSiZiWan; TP: triptolide; EV: estradiol valerate; PINK1: PTEN-induced kinase 1; SOD: superoxide dismutase; MDA: malondialdehyde; *p < 0.05 compared to the control group, #p < 0.05 compared to the TP group, &p < 0.05 compared to the TP + XJCRTSZW-High group. Source: Elaborated by the authors.
XJCRTSZW: XinJiaCongRongTuSiZiWan; TP: triptolide; EV: estradiol valerate; PINK1: PTEN-induced kinase 1; MMP: mitochondrial membrane potential; *p < 0.05 compared to the control group, #p < 0.05 compared to the TP group, &p < 0.05 compared to the TP + XJCRTSZW-High group. Source: Elaborated by the authors.