This research analyzes histologic alterations in allogenic bone graft from rats wich were prepared in two different ways. In one group (n=6), metatarsic-l bones not descalcified were stored at -70°C. In the other (n=6), the bones were desminerlised and freeze-drying. The bones were grafted into the groin region of rats and studied 30 days after. In the macroscopic evaluation, the bones not descalcified and stored at -70°C were surrounded by a thin capsule with consistency of cortical bone. Microscopically, it was observed reabsorption of endostal area with a decrease in the cortical thickness, and increase in the intracortical cavity. The bones descalcified and freeze-drying, in a macroscopic evaluation, were surrounded by a thick capsule and the consistency of the bone was softened. Microscopically, the bone showed a tissue without cells and vases, wich was very similar to cartilage; and a thick and fibrous capsule with inflamatory cells. Therefore, there were histologic differences in the allogenic bone grafts, and these differences depend on the way the bone was prepared and stored.
Freeze drying; Bone transplantation; Osseointegration; Rats
