OBJECTIVE: As little is known about the use of basic Fibroblast Growth Factor (FCFb) in the healing of abdominal aponeurosis, an experimental work was done to study it. METHODS: Twenty Wistar rats randomly separated in two groups were used. They were anesthetised with pentobarbital 20 mg/Kg intraperitonealy, submited to a 4cm median laparotomy and the aponeurosis was sutured with nylon 5-0. In group I 5mg of FCFb was applied over the sutured aponeurosis end the group II received saline 0,9% over it. After seven days the rats were killed by an overdose of anesthetic. A transversal 1,5 cm wideness sample of aponeurosis was submited to tensil strenght test, using the assay device EMIC MF500. Biopsies from the sutured tissue were processed and colored by HE and Masson trichromic. The histopatologic data were quantitated and transformed into mean density by the digital equipment Image Pro-plus. The Student t test was used and the differences were significant when p<0,05. RESULTS: In group I the the mean tensil strength was 1.103± 103,39gf and the mean density of histopatologic data was 226± 29,32. In group II (control) the tensil strenght was 791,1± 92, 77gf and the histolopatogical density was 114,1± 17,01. The differences were statisticaly significant (p<0,01). CONCLUSION: In conclusion, our data show that FCFb induces an increase of tensil strenght of sutured aponeurosis of rats and turns better the histopatological parameters of wound healing.
Fibroblast Growth Factor; Healing; Abdominal aponeurosis
