Animal model of chronic kidney disease using a unilateral technique of renal ischemia and reperfusion in White New Zealand rabbits¹

PURPOSE:

To establish a model of chronic kidney disease in White New Zealand rabbits, using an exclusive unilateral technique of renal ischemia and reperfusion.

METHODS:

Twenty males White New Zealand rabbits were used. All animals were subjected to the following: pre-surgical blood collection (1^st collection) for creatinine and urea serum analysis, left renal ischemia and reperfusion surgery technique, another blood sample was collected after 6 weeks post surgery (2^nd collection), the last blood sample (3^rd collection) blood sample was taken 11 weeks post surgery (pre-euthanasia), euthanasia and withdrawal of right and left kidney for histopathological analysis.

RESULTS:

The creatinine levels after surgery was statistically significant higher in the 3^rd collection, regarding the 1^st and 2^ndcollection (p<0.05). A significant statistic increase for urea was showed only in the 2^nd collection (p<0.05) when compared to the 1^stand 3^rd collections. Histopathological analysis showed bilateral lesions in the renal tissue, consistent to the process of ischemia and reperfusion.

CONCLUSION:

This exclusive unilateral technique of renal ischemia and reperfusion without nephrectomy in White New Zealand rabbits, showed effectiveness in getting an animal model of chronic kidney disease

Renal Insufficiency, Chronic; Models, Animal; Rabbits