Preconditioning with mono and polyunsaturated fatty acids and
               low-intensity electrical stimulation. Effects on skin repair in rats

Cardoso, Maria dos Prazeres Carneiro; Albuquerque, Andréa de Oliveira; Girão, Virginia Claudia Carneiro; Pompeu, Margarida Maria de Lima; Silva, Cícero Igor Simões Moura; Azevedo, Orleâncio Gomes Ritardo de; Guimarães, Sergio Botelho; Vasconcelos, Paulo Roberto Leitão de

doi:10.1590/S0102-86502015002000004

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Original Articles • Acta Cir. Bras. 30 (2) • Feb 2015 • https://doi.org/10.1590/S0102-86502015002000004 copy

Preconditioning with mono and polyunsaturated fatty acids and low-intensity electrical stimulation. Effects on skin repair in rats¹ 1Research performed at Experimental Surgery Laboratory (LABCEX), Department of Surgery, Faculty of Medicine, Federal University of Ceara (UFC), Fortaleza-CE, Brazil. Part of Master degree thesis, Postgraduate Program in Surgery, UFC. Tutor: Prof. Dr. Paulo Roberto Leitão de Vasconcelos.

Authorship SCIMAGO INSTITUTIONS RANKINGS

PURPOSE:

To evaluate the effects of preconditioning with oils mixes containing ω3/ω6/ω9 associated with micro-currents on skin repair in rats.

METHODS:

One-hundred and eight Wistar rats randomized into G-1, G-2 and G-3 groups were treated with saline (0.9%), mix 1 (corn+soybean oils) and mix 2 (olive+canola+flaxseed oils), respectively, in a single dose (0.01ml/g) by gavage. Next, each group was subdivided into sham and stimulated subgroups. Pulsed-wave microcurrents (0.5 µA, 0.5 Hz) were applied to stimulated subgroups for 20 min. One hour later anesthetized rats were subjected to surgery. A dorsal incision (6 cm long) was carried out and closed with interrupted nylon sutures. Samples (1cm²⁾ were harvested from the mid-portion of the incision on the 7, 14, 21 post-operative (P.O.) days. Variables were analyzed using Mann-Whitney/Dunn tests Significance level was set to 5 % (p<0.05).

RESULTS:

Micro-currents promoted increase of exudate and reduction of epithelialization on day 7 in G1 rats. Mixes 1/2 reduced vascularization on 7/14th days P.O. Both 1/2 mixes reduced fibrosis on day 14. Preconditioning with mix 1 led to increased expression of NF-kB on the 7th day.

CONCLUSION:

Preconditioning with microcurrents has pro-inflammatory effects while oil mixes 1 and 2 decrease fibrosis and vascularization in the proliferative phase of cicatrization.

Oils; Electric Stimulation Therapy; Wound Healing; Skin; Rats

Groups	Sub-groups	n	Treatments	Electric stimulation
G1 n=36	NS	18	saline 0.9%, single dose 0.01ml/g by gavage	none
G1 n=36	ST	18	saline 0.9%, single dose 0.01ml/g by gavage	Pulsed-wave microcurrents (0.5 µA, 0.5 Hz), for 20 min (single application)
G2 n=36	NS	18	corn+soybean oils [ω-6+ω--9], single dose 0.01ml/g) by gavage	none
G2 n=36	ST	18		Pulsed-wave microcurrents (0.5 µA, 0.5 Hz), for 20 min (single application)
G3 n=36	NS	18	olive+canola+flaxseed oils [ω-3+ω-6 + ω--9), single dose 0.01ml/g) by gavage	none
G3 n=36	ST	18		Pulsed-wave microcurrents (0.5 µA, 0.5 Hz), for 20 min (single application)

Time-point	EXUDATES			RE-EPITHELIALIZATION
	G1	G2	G3	G1	G2	G3
7^TH DAY	2 (1-2)	1.5 (1-2)*	2 (1-3)*	2 (1-2)	2 (1-2)**		2 (0-2)**
14^TH DAY	1.5 (1-3)	1 (1-2)	1 (1-2)	2 (0-2)	1 (0-2)		2 (1-2)
21^TH DAY	1 (1-2)	1 (1-1)	1.5 (1-3)	2 (2-2)	1 (0-2)		1.5 (0-2)

Time-point	FIBROSIS			VASCULARIZATION
	G1	G2	G3	G1	G2	G3
7^TH DAY	2 (1-2)	1 (1-2)	2 (1-2)	2 (1-3)	0.5 (0-2) *	2 (1-3)
14^TH DAY	2.5 (1-3)	1 (1-1) *	1 (1-1) *	2.5 (1-3)	1 (0-2)	0 (0-2) *
21^TH DAY	1.5 (1-2)	1 (1-2)	1 (1-3)	1 (1-1)	1 (0-2)	0 (0-3)

TP	G1		G2		G3
	SHAM	EE	SHAM	EE	SHAM	EE
7^TH DAY	2 (1-2)	3 (2-3)*	1.5 (1-2)	2 (1-3)	2 (1-3)	2 (1-3)
14^TH DAY	1.5 (1-3)	2 (1-3)	1 (1-2)	2 (1-2)	1 (1-2)	1 (1-2)
21^TH DAY	1 (1-2)	2 (1-3)	1 (1-1)	2 (1-3)	1.5 (1-3)	2 (1-3)

TP	G1		G2		G3
	SHAM	EE	SHAM	EE	SHAM	EE
7^TH DAY	2 (1-2)	0 (0-1) **	2 (1-2)	2 (0-2)	2 (0-2)	2 (0-2)
14^TH DAY	2 (0-2)	2 (2-2)	1 (0-2)	2 (2-2)	2 (1-2)	2 (2-2)
21^TH DAY	2 (2-2)	0 (0-1)	1 (0-2)	1 (0-2)	1,5 (0-2)	1,5 (0-2)

	G1	G2	G3
HSP27	88.80 ± 7.01	76.60 ± 13.55	73.00 ± 17.12
NF-kB	23.50 (17 – 61)	87.50 (23 – 198)*	72.00 (14 – 110)
NT	77.50 ± 15.21	76.20 ± 14.43	87.70 ± 16.08

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[1] Correspondence: Prof. Dr. Paulo Roberto Leitão de Vasconcelos Rua Prof. Costa Mendes, 1608/3º andar, Bloco Didático 60430-140 Fortaleza - CE Brasil Tel.: (55 85)3366-8063 paulolleitao@gmail.com