(+)-Catechin and (-)-epigallocatechin gallate: are these promising antioxidant therapies for frozen goat semen?

Silva, E.C.B.; Arruda, L.C.P.; Vieira, J.I.T.; Soares, P.C.; Guerra, M.M.P.

doi:10.1590/1678-4162-10539

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Veterinary Medicine • Arq. Bras. Med. Vet. Zootec. 71 (02) • Mar-Apr 2019 • https://doi.org/10.1590/1678-4162-10539 copy

(+)-Catechin and (-)-epigallocatechin gallate: are these promising antioxidant therapies for frozen goat semen?

(+)-Catequina e (-)-epigalocatequina galato: essas são promissoras terapias antioxidantes para a congelação de sêmen caprino?

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

The aim of this study was to evaluate the effects of different concentrations of (+)-catechin or (-)-epigallocatechin gallate (EGCG) on goat semen freezability. Poolsof semen were processed (Experiment 1: 0, 15, 25, 50, 75, or 100µM (+)-catechin; Experiment 2: 0, 15, 25, 50, 75, or 100µM EGCG) and frozen. After thawing, the samples were evaluated for kinematics, plasma membrane (PMi) and acrosome integrity, morphology, and oxidative stress, at 0 and 1h. In Experiment 1, at 0h, VSL and VAP were greater (P<0.05) with 15µM than with 50 and 100; WOB was lower (P<0.05) with 100µM than with 0, 15, and 25; and BCF was higher (P<0.05) with 75 and 100µM than with 0. In turn, in Experiment 2, progressive motility was higher (P<0.05) with0 and 15µM than with50 and 75; LIN was lower (P<0.05) with75 and100µM than with0 and 15; WOB was higher (P<0.05) with0 and 15µM; and PMi was greater (P<0.05) with100µM than 0. Thus, (+)-catechin or EGCG at higher concentrations inhibits the kinematics of frozen goat sperm, in a transitory way, and 100µM of EGCG preserves the PMi.

Keywords:
antioxidants; cryopreservation; flavonoids; oxidative stress; semen

Universidade Federal de Minas Gerais, Escola de Veterinária Caixa Postal 567, 30123-970 Belo Horizonte MG - Brazil, Tel.: (55 31) 3409-2041, Tel.: (55 31) 3409-2042 - Belo Horizonte - MG - Brazil
E-mail: abmvz.artigo@gmail.com

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[1] E-mail: silva.ecb@gmail.com

		Concentrations of (+)-catechin						Regression assay*
Parameters	Time							P>F
		0µM	15µM	25µM	50µM	75µM	100µM	L	Q	C
TM	0h	68.2±7.0	71.0±13.8	71.2±9.4	69.4±7.2	67.2±10.3	72.1±10.7	Ns	ns	Ns
	1h	59.5±11.5	54.3±15.8	56.0±14.6	57.9±8.6	57.1±14.4	56.0±15.1	Ns	ns	Ns
PM	0h	23.7±3.7	27.7±4.1	24.2±2.6	21.8±2.3	23.5±3.2	21.2±4.6	Ns	ns	Ns
	1h	25.1±3.6	23.3±9.6	21.8±5.5	23.1±8.8	23.3±6.4	21.0±12.2	Ns	ns	Ns
VCL	0h	81.2±10.1	86.0±4.6	80.9±2.5	75.7±7.0	80.3±10.8	77.4±8.1	Ns	ns	Ns
	1h	78.1±7.5	80.4±15.2	77.1±13.1	76.8±14.6	79.0±11.4	73.8±12.2	Ns	ns	Ns
VSL	0h	41.7±5.3^ab	45.4±5.3^a	41.5±3.7^ab	36.9±1.4^b	39.7±3.5^ab	35.2±3.1^b	0.0008	ns	Ns
	1h	42.4±4.0	43.2±6.1	41.5±7.2	40.4±8.0	41.3±2.4	38.0±8.8	Ns	ns	Ns
VAP	0h	55.4±7.2^ab	59.2±4.4^a	54.7±3.1^ab	49.3±2.6^b	52.2±6.3^ab	48.0±4.5^b	0.0012	ns	Ns
	1h	52.2±4.6	53.8±9.2	52.4±9.1	50.5±9.9	51.4±5.2	47.6±9.4	Ns	ns	ns
LIN	0h	51.4±2.9	52.9±7.0	51.3±3.9	49.2±5.1	49.9±4.7	45.6±2.2	0.0139	ns	ns
	1h	54.5±5.0	54.1±4.2	54.2±7.2	53.0±6.8	52.8±4.9	51.2±5.8	Ns	ns	ns
STR	0h	75.3±2.5	76.7±6.4	75.9±2.8	75.0±4.5	76.4±4.6	73.4±3.0	Ns	ns	ns
	1h	81.2±3.4	80.6±3.6	79.3±5.0	80.2±6.0	80.8±4.1	79.6±6.3	Ns	ns	ns
WOB	0h	68.2±2.5^a	68.8±4.2^a	67.5±2.8^a	65.4±3.2^ab	65.2±2.3^ab	62.1±1.2^b	<.0001	ns	ns
	1h	67.0±3.9	67.1±3.3	68.1±5.7	65.9±4.7	65.3±3.4	64.2±3.0	ns	ns	ns
ALH	0h	3.1±0.2	3.2±0.2	3.1±0.2	3.1±0.3	3.2±0.4	3.3±0.2	ns	ns	ns
	1h	3.2±0.2	3.2±0.4	3.1±0.4	3.1±0.5	3.2±0.3	3.2±0.3	ns	ns	ns
BCF	0h	11.1±0.8^c	11.4±0.6^abc	11.3±0.4^ab	11.7±0.5^abc	12.4±0.5^a	12.2±0.8^ab	0.0003	ns	ns
	1h	13.0±1.0	12.8±1.2	12.5±0.8	13.2±0.9	13.1±1.1	13.4±1.3	ns	ns	ns

Parameters	Time	Concentrations of EGCG						Regression assay*
Parameters	Time							P>F
		0µM	15µM	25µM	50µM	75µM	100µM	L	Q	C
TM	0h	68.8±9.0	66.4±11.5	67.4±11.8	60.0±11.0	59.7±10.7	69.1±8.3	ns	ns	ns
	1h	59.5±7.4	49.8±16.1	51.3±7.3	57.6±15.2	55.4±19.5	54.4±17.4	ns	ns	ns
PM	0h	27.1±2.6^a	27.2±4.3^a	24.4±2.6^ab	19.4±2.8^b	19.9±2.0^b	24.6±6.6^ab	0.0035	ns	ns
	1h	24.2±9.1	19.7±6.2	18.4±3.6	19.7±6.3	21.1±7.4	19.8±5.9	ns	ns	ns
VCL	0h	80.4±14.6	84.3±9.6	81.0±7.7	78.1±11.7	82.7±9.4	84.1±6.7	ns	ns	ns
	1h	75.6±12.2	71.3±6.3	68.7±5.9	72.4±13.7	75.1±9.8	78.0±12.8	ns	ns	ns
VSL	0h	43.9±6.1	45.8±5.9	41.8±3.7	38.3±6.1	39.6±6.2	39.6±5.4	0.0269	ns	ns
	1h	42.0±12.8	38.5±6.2	35.9±3.3	35.8±4.6	38.8±6.0	39.0±4.5	ns	ns	ns
VAP	0h	56.1±9.0	58.6±7.7	55.1±5.8	50.8±8.6	53.3±7.5	53.5±4.9	ns	ns	ns
	1h	52.0±12.1	47.0±5.0	45.3±2.9	45.9±7.4	48.6±6.2	49.9±7.0	ns	ns	ns
LIN	0h	55.1±5.2^a	54.4±3.5^ab	51.7±3.2^abc	49.1±3.2^abc	47.8±3.7^bc	47.1±5.9^c	<.0001	ns	ns
	1h	54.6±8.6	54.1±7.9	52.4±5.4	50.1±5.3	51.8±7.1	50.6±5.5	ns	ns	ns
STR	0h	78.7±4.2	78.5±3.8	76.1±4.2	75.5±3.8	74.3±3.4	73.8±4.7	0.0085	ns	ns
	1h	79.7±6.2	81.5±6.0	79.2±5.1	78.4±4.1	79.6±5.4	78.6±5.7	ns	ns	ns
WOB	0h	70.0±3.1^a	69.2±2.0^a	67.9±1.6^ab	65.0±2.3^b	64.3±2.5^b	63.7±3.8^b	<.0001	ns	ns
	1h	68.2±5.8	66.0±5.4	66.1±2.8	63.8±3.5	64.9±4.8	64.3±2.3	ns	ns	ns
ALH	0h	3.0±0.4	3.0±0.2	3.1±0.2	3.3±0.2	3.2±0.1	3.2±0.3	0.0271	ns	ns
	1h	3.0±0.2	3.0±0.3	3.1±0.3	3.1±0.4	3.0±0.4	3.2±0.3	ns	ns	ns
BCF	0h	11.5±0.9	12.2±1.0	12.1±0.7	12.1±1.0	12.2±0.8	12.4±0.6	ns	ns	ns
	1h	12.5±1.3	13.1±0.8	12.4±0.9	13.1±0.6	13.0±1.0	13.4±0.7	ns	ns	ns

Parameters	Time	Concentrations of (+)-catechin						Regression assay*
Parameters	Time							P>F
		0µM	15µM	25µM	50µM	75µM	100µM	L	Q	C
PMi	0h	39.6±6.5	44.6±5.6	47.4±8.4	43.5±7.2	48.0±7.8	47.1±4.0	ns	ns	ns
	1h	28.1±6.7	32.8±8.7	34.0±7.3	33.4±5.6	37.2±12.2	33.6±7.4	ns	ns	ns
ACi	0h	50.9±9.3	48.3±4.3	48.8±4.3	53.2±10.1	52.5±5.5	49.7±9.0	ns	ns	ns
	1h	51.5±14.9	48.5±10.6	44.3±10.9	50.8±13.8	46.6±7.2	48.9±7.3	ns	ns	ns
nMOR	0h	79.9±3.2	79.6±3.8	78.4±2.5	75.8±4.9	78.3±4.0	77.0±2.7	ns	ns	ns
	1h	76.3±8.4	74.9±3.1	73.5±2.6	76.6±5.3	70.8±5.4	75.2±1.8	ns	ns	ns
-OE	0h	78.8±4.0	81.2±4.9	82.5±3.5	83.0±3.4	82.3±6.5	82.3±4.3	ns	ns	ns
	1h	76.3±4.8	76.5±3.3	75.2±5.1	78.8±4.1	77.0±4.5	80.2±5.9	ns	ns	ns

Parameters	Time	Concentrations of EGCG						Regression assay*
Parameters	Time							P>F
		0µM	15µM	25µM	50µM	75µM	100µM	L	Q	C
PMi	0h	39.3±4.7^b	44.5±6.8^ab	41.0±5.5^ab	43.5±8.2^ab	43.3±4.2^ab	51.3±8.3^a	0.0117	ns	ns
	1h	37.6±6.6	34.8±10.0	36.1±9.9	31.5±13.0	31.6±7.2	33.0±5.0	ns	ns	ns
ACi	0h	44.8±7.0	45.1±8.1	47.0±10.5	46.0±6.6	47.8±4.9	49.7±7.6	ns	ns	ns
	1h	43.4±8.8	42.7±11.8	39.6±9.7	42.1±10.2	40.0±11.0	42.1±9.8	ns	ns	ns
nMOR	0h	86.6±5.3	84.5±6.3	84.5±6.8	84.8±6.4	87.5±1.8	84.4±6.9	ns	ns	ns
	1h	81.6±10.4	84.9±6.4	82.3±8.6	80.3±10.5	81.8±10.1	79.8±11.5	ns	ns	ns
-OE	0h	78.5±3.5	81.0±4.4	82.3±2.8	82.7±4.6	81.3±4.0	82.0±2.9	ns	ns	ns
	1h	74.0±2.9	76.0±4.6	78.7±4.7	78.0±3.9	78.8±2.2	77.2±2.9	ns	ns	ns