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Comparison between "in vitro" methods to determine the bioavaiability of phosphorus

Dicalcium phosphate (FB), monoammonium phosphate (MAP), monocalcium phosphate (FST), rock phosphate from Araxá (FRA), calcinated bone meal (FOC) and steamed bone meal (FOA) were used to study phosphorus solubility by the following extractors: water, citric acid at concentrations of 2% (AC2), 10% (AC10), 20% (AC20) and 30% (AC30), hydrochloric acid at 0.5% (HCL) and ammonium neutral citrate (CAN) in standardized laboratory condition. The FB phosphorus water solubility was 33.8% while the FOC, FOA and FRA phosphorus were practically insoluble in water. The FOC and FRA phosphorus HCL solubility was respectively 42.7 and 42.9%. The FOA phosphorus solubility in CAN was 47.1% but the FOC and FRA were not soluble. The FOC and FRA phosphorus solubility in AC2 extractor were 38.7 and 20.8% while those for FRA phosphorus were 53.2 and 59.5%, respectively. The best results were observed for phosphorus solubility using AC extractors. They can be used as a guide for the biological value of phosphorus from the studied sources based on the FRA phosphorus solubility (44.6) and the 82.2%-100% phosphorus solubility from the other studied sources.

phosphorus; solubility; extractor; citric acid


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