[Estudos morfológicos e moleculares sobre um parasita nematoide, Syphacia obvelata, que infecta camundongos de laboratório (Mus musculus) na Arábia Saudita]

Mohammed, O.B.; Al-Shaebi, E.M.; Omer, S.A.; Abdel-Gaber, R.A.

O.B. Mohammed

Department of Zoology, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia

http://orcid.org/0000-0001-5457-2555

E.M. Al-Shaebi

Department of Zoology, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia

http://orcid.org/0000-0003-1614-4802

S.A. Omer

Department of Zoology, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia

http://orcid.org/0000-0001-7922-9411

R.A. Abdel-Gaber ^** Corresponding author: rabdelgaber.c@ksu.edu.sa

Department of Zoology, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia

http://orcid.org/0000-0001-9263-6871

^* Corresponding author: rabdelgaber.c@ksu.edu.sa

Figuras (3)
Tabelas (2)

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Figure 1
Line drawing of Syphacia obvelata infecting laboratory mice. (A) Lateral view of male, holotype. (B) Lateral view of female, holotype. (C) Lateral view of embryonated egg. (D) Lateral view of mamelon. (E) Ventral view of the posterior end of male. (F) Ventral view of the spicule. Note: ACP, ad-cloacal papillae; AN, anal opening; B, bulb; C, corpus; CO, cloacal opening; EG, eggs; EP, excretory pore; GN, gubernaculum; IN, intestine; L, lips; LP, lateral papillae; M, mamelon; NR, nerve ring; OA, ovijector apparatus; OP, operculum; PCP, pre-cloacal papillae; POCP, post-cloacal papillae; R, rectum; SP, spicule; T, tail; TE, testis; VA, valvular apparatus; VL, vulval lips.

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Figure 2
A consensus phylogenetic tree constructed with maximum likelihood (ML) and Neighbor Joining (NJ) methods, showing phylogenetic relationships among Syphacia obvelata and other Syphacia species and related taxa in NCBI GenBank with Clonorchis sinensis as an outgroup. The ML and NJ trees are inferred from the 18S rDNA sequence data generated from the S. obvelata recovered from Mus musculus (OQ875250 given in bold) and related taxa from GenBank. Numbers indicated at branch nodes are bootstrap values (ML/NJ). Only bootstraps > 70% are shown.

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Figure 3
A consensus phylogenetic tree constructed with maximum likelihood (ML) and Neighbor Joining (NJ) methods, showing phylogenetic relationships among Syphacia obvelata and other Syphacia species and related taxa in NCBI GenBank with Clonorchis sinensis as an outgroup. The ML and NJ trees are inferred from the ITS spacer regions (ITS1, 5.8S, and ITS2) rDNA sequence data generated from the S. obvelata recovered from Mus musculus (OQ874775 given in bold) and related taxa from GenBank. Numbers indicated at branch nodes are bootstrap values (NJ /ML). Only bootstraps > 60% are shown.

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Table 1
Morphological characteristics of male worms for Syphacia obvelata from laboratory mice

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Table 2
Morphological characteristics of female worms for Syphacia obvelata from laboratory mice

Source of Syphacia	Body		Esophagus		Distance from anterior end		Spicule length	Tail length	Locality
Source of Syphacia	Length	Width	Corpus	bulb	Nerve ring	Excretory pore
Hussey, 1957HUSSEY, K. Syphacia muris vs. Syphacia obvelata in laboratory rats and mice. J. Parasitol., v.43, p.555-559, 1957.	1.334	0.131	--	--	--	0.302	--	0.122	USA
Ogden, 1971OGDEN, C. Observations in the sistematic of nematodes belonging to the genus Syphacia Seurat, 1916. Bull. Br. Mus., v.20, p.255-291, 1971.	1.13-1.61 (1.35)	0.131-0.172 (0.146)	--	--	--	--	0.068-0.089 (0.078)	0.122-0.172 (0.149)	Several countries
*Magalhaes et al., 1994*MAGALHAES, R.; VICENTE, J.; NOROÑA, D. Helminth parasites of conventionally maintained laboratory mice. Mem. Inst. Oswaldo Cruz, v.89, p.33-40, 1994.	1-1.1	0.050-0.072	--	--	0.090-0.097	0.079	0.072-0.082	0.072-0.090	Brazil
Landaeta-Aqueveque et al., 2007	0.88-1.23 (1.04)	0.120-0.190 (0.150)	--	0.050-0.067 (0.060)	0.090-0.120 (0.100)	0.170-0.220 (0.190)	0.055-0.085 (0.077)	0.110-0.170 (0.130)	Chile
*Khalil et al., 2014*KHALIL, A.I.; LASHEIN, G.H.; MORSY, G.H.; ABD EL-MOTTALEB, D.I. Oxyurids of wild and laboratory rodents from Egypt. Life Sci. J., v.11, p.94-107, 2014.	0.432-1.09 (0.677)	0.067-0.150 (0.108)	0.016-0.045 (0.037)	0.027-0.090 (0.047)	0.040-0.126 (0.065)	0.099-0.220 (0.134)	0.058-0.097 (0.073)	0.094-0.117 (0.105)	Egypt
Abdel-Gaber, 2016	0.623-1.130 (0.830)	0.092-0.130 (0.110)	0.013-0.042 (0.030)	0.023-0.086 (0.053)	0.035-0.132 (0.073)	0.087-0.191 (0.145)	0.042-0.082 (0.054)	0.073-0.142 (0.120)	Egypt
Present study	0.72-1.11 (0.90)	0.10-0.12 (0.11)	0.02-0.05 (0.04)	0.03-0.10 (0.07)	0.05-0.13 (0.08)	0.09-0.20 (0.17)	0.05-0.09 (0.07)	0.08-0.16 (0.14)	Saudi Arabia

Source of Syphacia	Body		Esophagus		Distance from anterior end			Eggs		Tail length	Locality
Source of Syphacia	Length	Width	Corpus	bulb	Nerve ring	Excretory pore	Vulva opening	Length	Width
Hussey, 1957HUSSEY, K. Syphacia muris vs. Syphacia obvelata in laboratory rats and mice. J. Parasitol., v.43, p.555-559, 1957.	5.203	0.312	--	--	--	0.523	0.874	0.134	0.036	0.762	USA
Ogden, 1971OGDEN, C. Observations in the sistematic of nematodes belonging to the genus Syphacia Seurat, 1916. Bull. Br. Mus., v.20, p.255-291, 1971.	3.72-5.61 (4.69)	0.234-0.372 (0.316)	--	--	--	--	0.450-0.740 (0.590)	0.099-0.118	0.036-0.041	0.680-0.890 (0.780)	Several countries
*Magalhaes et al., 1994*MAGALHAES, R.; VICENTE, J.; NOROÑA, D. Helminth parasites of conventionally maintained laboratory mice. Mem. Inst. Oswaldo Cruz, v.89, p.33-40, 1994.	4.5-5	0.245-0.350	--	--	0.082-0.090	0.086	0.662-0.806	0.118-0.126	0.043-0.054	0.518	Brazil
Landaeta-Aqueveque et al., 2007	3.7-4.8 (4.28)	0.290-0.400 (0.320)	--	0.085-0.126 (0.111)	0.115-0.125 (0.120)	0.250-0.510 (0.350)	0.430-0.790 (0.550)	0.132-0.140 (0.136)	0.031-0.050 (0.040)	0.600-0.770 (0.670)	Chile
*Khalil et al., 2014*KHALIL, A.I.; LASHEIN, G.H.; MORSY, G.H.; ABD EL-MOTTALEB, D.I. Oxyurids of wild and laboratory rodents from Egypt. Life Sci. J., v.11, p.94-107, 2014.	1.443-3.08 (1.96)	0.13-0.252 (0.166)	0.054-0.081 (0.066)	0.045-0.118 (0.069)	0.063-0.14 (0.082)	0.175-0.369 (0.251)	0.279-0.585 (0.401)	0.087-0.11 (0.103)	0.022-0.027 (0.024)	0.319-0.576 (0.455)	Egypt
Abdel-Gaber, 2016	2.930-4.650 (3.540)	0.120-0.232 (0.156)	0.018-0.062 (0.051)	0.049-0.156 (0.110)	0.026-0.157 (0.121)	0.134-0.243 (0.195)	0.323-0.632 (0.546)	0.120-0.139 (0.129)	0.030-0.052 (0.045)	0.220-0.410 (0.361)	Egypt
Present study	3.12-5.01 (4.30)	0.13-0.29 (0.17)	0.02-0.06 (0.05)	0.05-0.17 (0.12)	0.03-0.15 (0.13)	0.14-0.20 (0.18)	0.40-0.60 (0.58)	0.07-0.09 (0.08)	0.03-0.05 (0.04)	0.22-0.40 (0.34)	Saudi Arabia

[1] ^* Corresponding author: rabdelgaber.c@ksu.edu.sa

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[Estudos morfológicos e moleculares sobre um parasita nematoide, Syphacia obvelata, que infecta camundongos de laboratório (Mus musculus) na Arábia Saudita]

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