Red cabbage <i>(Brassica oleracea</i> L.) extract reverses lipid oxidative stress in rats

Red cabbage (Brassica oleracea L. var. capitata f. rubra DC.) extract has been demonstrated hypolipidemic and antioxidant capacity. Herein, we investigated the effect of red cabbage aqueous extract (RC) or fenofibrate (FF) in oxidative stress induced by Triton WR-1339 in rats. The antioxidant capacity was evaluated through the superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities and, thiobarbituric reactive species (TBARS) and protein carbonyl (PC) levels in erythrocytes, liver, kidneys, cerebral cortex and hippocampus of male rats. The alterations promoted by Triton WR-1339 in enzymatic antioxidant defense in the liver, kidneys and hippocampus were reversed by RC or FF treatments. The TBARS and PC levels increased in the liver, cerebral cortex and hippocampus of hyperlipidemic rats were decreased by the treatments with RC or FF. These findings demonstrated that RC is a potential therapy to treat diseases not only involving dyslipidemic condition but also oxidative stress.

Key words
red cabbage; Brassica oleracea; oxidative stress; antioxidants; Triton WR-1339

Authorship

BRUNO VEBER

Laboratório de Avaliação de Substâncias Bioativas, Departamento de Ciências Naturais, Universidade Regional de Blumenau, Rua Antônio da Veiga, 140, Victor Konder, 89030-903 Blumenau, SC, Brazil Universidade Regional de BlumenauBrazilBlumenau, SC, BrazilLaboratório de Avaliação de Substâncias Bioativas, Departamento de Ciências Naturais, Universidade Regional de Blumenau, Rua Antônio da Veiga, 140, Victor Konder, 89030-903 Blumenau, SC, Brazil

https://orcid.org/0000-0002-5206-3471

ANDERSON CAMARGO

https://orcid.org/0000-0002-1632-4865

ANA PAULA DALMAGRO

https://orcid.org/0000-0001-6969-7473

HENRIQUE LUIS P. BONDE

Laboratório de Biofísica, Departamento de Ciências Naturais, Universidade Regional de Blumenau, Rua Antônio da Veiga, 140, Victor Konder, 89030-903 Blumenau, SC, Brazil Universidade Regional de BlumenauBrazilBlumenau, SC, BrazilLaboratório de Biofísica, Departamento de Ciências Naturais, Universidade Regional de Blumenau, Rua Antônio da Veiga, 140, Victor Konder, 89030-903 Blumenau, SC, Brazil

https://orcid.org/0000-0002-3977-6717

DÉBORA D. DAL MAGRO

https://orcid.org/0000-0003-4459-1562

DANIELA D. DE LIMA

Departamento de Medicina, Universidade da Região de Joinville, Rua Paulo Malschitzki, 10, Zona Industrial Norte, 89219-710 Joinville, SC, Brazil Universidade da Região de JoinvilleBrazilJoinville, SC, BrazilDepartamento de Medicina, Universidade da Região de Joinville, Rua Paulo Malschitzki, 10, Zona Industrial Norte, 89219-710 Joinville, SC, Brazil

https://orcid.org/0000-0001-5335-5102

ANA LÚCIA B. ZENI

https://orcid.org/0000-0002-9904-9861

Correspondence to Ana Lúcia Bertarello Zeni E-mail: anazeni@furb.br

AUTHOR CONTRIBUTIONS

BV performed experiments, collected samples, analyzed data and co-wrote the paper. AC and APD performed experiments and co-wrote the paper. BG collected samples, performed experiments and co-wrote the paper. DDDM and DDL supervised the research, designed experiments and co-wrote the paper. ALBZ supervised the research, designed experiments, analyzed data and co-wrote the paper.

SCIMAGO INSTITUTIONS RANKINGS

Figures

Figures (5)

Thumbnail

Figure 1
Effects of red cabbage aqueous extract (RC) and fenofibrate (FF) treatments on oxidative stress markers in kidneys (left, panels a and d), liver (middle, panels b and e) and serum (right, panels c and f) of Triton WR-1339-induced hyperlipidemia in rats. Bars represent means± S.D. (n=5–6). *p<0.05, **p<0.001, ***p<0.001 vs normal control (NC) and #p<0.05, ##p<0.01, ###p<0.001 vs Triton control (TC), according to one-way ANOVA followed by the Tukey’s post hoc test. MDA= malondialdehyde.

Thumbnail

Figure 2
Effects of red cabbage aqueous extract (RC) and fenofibrate (FF) treatments on oxidative stress markers in cerebral cortex (left, panels a and c) and hippocampus (right, panels b and d) of Triton WR-1339-induced hyperlipidemia in rats. Bars represent means± S.D. (n=5–6). **p<0.001, ***p<0.001 vs normal control (NC) and #p<0.05, ##p<0.01, ###p<0.001 vs Triton control (TC), according to one-way ANOVA followed by Tukey’s test. MDA= malondialdehyde.

Thumbnail

Figure 3
Effects of red cabbage aqueous extract (RC) and fenofibrate (FF) treatments on antioxidant enzymes activities in kidneys (left, panels a, d and g), liver (middle, panels b, e and h) and erythrocytes (right, panels c, f and i) of Triton WR-1339-induced hyperlipidemia in rats. Bars represent means± S.D. (n=5–6). **p<0.001, ***p<0.001 vs normal control (NC) and ##p<0.01, ###p<0.001 vs Triton control (TC), according to one-way ANOVA followed by Tukey’s test. SOD= superoxide dismutase; CAT= catalase; GSH-Px= glutathione peroxidase.

Thumbnail

Figure 4
Effects of red cabbage aqueous extract (RC) and fenofibrate (FF) treatments on antioxidant enzymes activities in cerebral cortex (left, panels a, c and e) and hippocampus (right, panels b, d and f) of Triton WR-1339-induced hyperlipidemia in rats. Bars represent means± S.D. (n=5–6). ***p<0.001 vs normal control (NC) and ###p<0.001 vs Triton control (TC), according to one-way ANOVA followed by Tukey’s test. SOD= superoxide dismutase; CAT= catalase; GSH-Px= glutathione peroxidase.

Thumbnail

Figure 5
The schematic diagram shows biochemical pathways underlying Triton WR-1339-induced lipid oxidative stress and the antioxidant effects of RC and FF, a PPARα agonist. In this study, the RC and FF counteracted the alterations induced by Triton beyond the decrement of TBARS and PC levels normalizing the enzyme activities. CAT=catalase; FF= fenofibrate; GSH-Px= glutathione peroxidase; LPL= lipoprotein lipase; PPARα= Peroxisome proliferator-activated receptor alpha; PC= protein carbonyl; RC= red cabbage aqueous extract; SOD= superoxide dismutase; TBARS=thiobarbituric acid reactive substances.

Figure 1 Effects of red cabbage aqueous extract (RC) and fenofibrate (FF) treatments on oxidative stress markers in kidneys (left, panels a and d), liver (middle, panels b and e) and serum (right, panels c and f) of Triton WR-1339-induced hyperlipidemia in rats. Bars represent means± S.D. (n=5–6). p<0.05, p<0.001, p<0.001 vs normal control (NC) and #p<0.05, ##p<0.01, ###p<0.001 vs Triton control (TC), according to one-way ANOVA followed by the Tukey’s post hoc test. MDA= malondialdehyde.

Figure 2 Effects of red cabbage aqueous extract (RC) and fenofibrate (FF) treatments on oxidative stress markers in cerebral cortex (left, panels a and c) and hippocampus (right, panels b and d) of Triton WR-1339-induced hyperlipidemia in rats. Bars represent means± S.D. (n=5–6). p<0.001, *p<0.001 vs normal control (NC) and #p<0.05, ##p<0.01, ###p<0.001 vs Triton control (TC), according to one-way ANOVA followed by Tukey’s test. MDA= malondialdehyde.

Figure 3 Effects of red cabbage aqueous extract (RC) and fenofibrate (FF) treatments on antioxidant enzymes activities in kidneys (left, panels a, d and g), liver (middle, panels b, e and h) and erythrocytes (right, panels c, f and i) of Triton WR-1339-induced hyperlipidemia in rats. Bars represent means± S.D. (n=5–6). p<0.001, *p<0.001 vs normal control (NC) and ##p<0.01, ###p<0.001 vs Triton control (TC), according to one-way ANOVA followed by Tukey’s test. SOD= superoxide dismutase; CAT= catalase; GSH-Px= glutathione peroxidase.

Figure 4 Effects of red cabbage aqueous extract (RC) and fenofibrate (FF) treatments on antioxidant enzymes activities in cerebral cortex (left, panels a, c and e) and hippocampus (right, panels b, d and f) of Triton WR-1339-induced hyperlipidemia in rats. Bars represent means± S.D. (n=5–6). ***p<0.001 vs normal control (NC) and ###p<0.001 vs Triton control (TC), according to one-way ANOVA followed by Tukey’s test. SOD= superoxide dismutase; CAT= catalase; GSH-Px= glutathione peroxidase.

Figure 5 The schematic diagram shows biochemical pathways underlying Triton WR-1339-induced lipid oxidative stress and the antioxidant effects of RC and FF, a PPARα agonist. In this study, the RC and FF counteracted the alterations induced by Triton beyond the decrement of TBARS and PC levels normalizing the enzyme activities. CAT=catalase; FF= fenofibrate; GSH-Px= glutathione peroxidase; LPL= lipoprotein lipase; PPARα= Peroxisome proliferator-activated receptor alpha; PC= protein carbonyl; RC= red cabbage aqueous extract; SOD= superoxide dismutase; TBARS=thiobarbituric acid reactive substances.

How to cite

copy

Academia Brasileira de Ciências Rua Anfilófio de Carvalho, 29, 3º andar, 20030-060 Rio de Janeiro RJ Brasil, Tel: +55 21 3907-8100 - Rio de Janeiro - RJ - Brazil
E-mail: aabc@abc.org.br

Acompanhe os números deste periódico no seu leitor de RSS

Versão para download de PDF

PDF

Inglês

Versões e tradução automática

Versão original do texto

English

Tradução automática

Google Translator
Microsoft Translator

Como citar

RIS

BIBTEX

Outros formatos de citação e exportação:

SciELO - Scientific Electronic Library Online
Rua Dr. Diogo de Faria, 1087 – 9º andar – Vila Clementino 04037-003 São Paulo/SP - Brasil
E-mail: scielo@scielo.org

Leia a Declaração de Acesso Aberto

Métricas

Red cabbage <i>(Brassica oleracea</i> L.) extract reverses lipid oxidative stress in rats

PlumX

Mensagem

[1] Correspondence to Ana Lúcia Bertarello Zeni E-mail: anazeni@furb.br