Kinkan orange protects hypercholesterolemic rats against dyslipidemia and oxidative stress

SILVA, DAYSE LÚCIA; SILVA, NICOLLE CAMILLA R. DA; AGUILAR, EDENIL C.; SILVA, MARCELO EUSTÁQUIO; OLIVEIRA, DIRCE R. DE

doi:10.1590/0001-3765202220201066

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HEALTH SCIENCES • An. Acad. Bras. Ciênc. 94 (suppl 3) • 2022 • https://doi.org/10.1590/0001-3765202220201066 copy

Kinkan orange protects hypercholesterolemic rats against dyslipidemia and oxidative stress

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

We investigated the effect of dietary supplementation with kinkan orange on growth, adiposity, metabolic parameters, and oxidative stress in rats with diet-induced hypercholesterolemia. Female Wistar rats (6-8 weeks) were fed a AIN-93M diet (Control); AIN-93M diet containing 5% kinkan orange (CTkinkan); Hypercholesterolemic diet, containing 1% cholesterol and 25% fat (Hyper); or Hypercholesterolemic diet containing 5% kinkan orange (Hyperkinkan). Hypercholesterolemic diet increased body weight, adiposity, serum alanine transaminase (ALT), creatinine, cholesterol and triglycerides, hepatic total lipids, cholesterol, and triglycerides, and hepatic oxidative stress. Supplementation with kinkan reduced the serum and hepatic lipid content, decreased serum ALT, besides improving the antioxidant status in liver tissue of hypercholesterolemic animals. Moreover, HDL-cholesterol increased in both groups supplemented with kinkan orange (CTkinkan and Hyperkinkan). Our data suggest that diet supplementation with kinkan orange may consist of a valid strategy to prevent or reduce dyslipidemia and oxidative stress in hypercholesterolemic rats.

Key words
citrus; dyslipidemia; high-fat diet; oxidative stress

	Experimental groups				Two-way Anova P val ue
Variables	Contro l	CTkinkan	Hyper	Hyperkinkan	Cholesterol	Kinkan	Cholesterol x Kinkan
Food intake (g/animal/day)	2.80 ± 0.21^a	2.78 ± 0.23^a	2.39 ± 0.31^a	2.80 ± 0.21^a	0.12	0.80	0.84
Energy intake (kcal/animal/day)	11.07 ± 0.83^a	11.09 ± 0.90^a	11.50 ± 1.50^a	10.93 ± 1.67^a	0.92	0.84	0.82
Final body weight (g)	222.8 ± 4.25^a	215.0 ± 4.04^a	252.5 ± 9.15^b	260.9 ± 2.80^b	<0.0001	0.96	0.17
Relative liver weight (%)	3.27 ± 0.11^a	3.17 ± 0.11^a	5.21 ± 0.14^b	5.12 ± 0.15^b	<0.0001	0.45	0.96
Adiposity index (%)	1.90 ± 0.13^a	1.90 ± 0.21^a	2.55 ± 0.13^b	2.60 ± 0.17^b	0.0003	0.89	0.84
Serum
AST (U/mL)	39.32 ± 3.00^a	28.96 ± 1.73^b	39.52 ± 5.52^a	27.48 ± 1.46^b	0.85	0.002	0.80
ALT (U/mL)	15.26 ± 0.53^a	13.99 ± 0.80^a	19.73 ± 2.18^a	8.38 ± 0.21^b	0.59	<0.0001	<0.0001
Urea (mmol/L)	3.98 ± 0.26^a	3.25 ± 0.17^b	3.50 ± 0.13^a	3.38 ± 0.24^b	0.27	0.03	0.25
Creatinin (mmol/L)	37.84 ± 2.86^b	30.84 ± 2.75^a	51.40 ± 2.60^b	43.27 ± 1.63^c	<0.0001	0.005	0.82
Cholesterol (mg/dL)	153.3 ± 14.45^a	146.6 ± 11.73^a	281.9 ± 26.97^b	157.6 ± 17.05^a	0.0007	0.001	0.004
Triglycerides (mg/dL)	115.5 ± 0.50^a	123.9 ± 4.92^a	159.6± 8.79^b	110.5 ± 7.44^a	0.03	0.005	<0.0001
Liver (mg/g tissue)
Total lipids	47.86 ± 4.14^a	39.50 ± 2.18^a	280.80 ± 12.67^b	233.10 ± 8.10^c	<0.0001	0.002	0.022
Cholesterol	2.16± 0.14^a	1.91 ± 0.12^a	24.55 ± 1.56^b	19.90 ± 1.04^c	<0.0001	0.015	0.03
Triglycerides	8.68 ± 1.20^a	1.84 ± 0.11^b	16.60 ± 0.66^c	12.76 ± 1.23^d	<0.0001	<0.0001	0.04

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[1] Correspondence to: Dirce Ribeiro de Oliveira E-mail: dirce.oliveira@ufjf.br