RESUMO: Na micropropagação, o nitrato de potássio (KNO3), reagente puro para análise (P.A.), utilizado no preparo dos meios de cultura, possui custo elevado e a sua aquisição depende de trâmites burocráticos, por se tratar de substância controlada pelo Exército Brasileiro. O objetivo deste trabalho foi avaliar o efeito da substituição do KNO3 P.A. por fertilizante comercial (com fonte de KNO3), encontrado livremente no comércio, na micropropagação de palma (Opuntia stricta (Haw.) Haw. cv Orelha de Elefante. Os tratamentos foram de seis concentrações do fertilizante (0; 0,5; 1; 1,5; 2 e 2,5 g L-1) e um controle constituído de 1,9 g L-1 de reagente KNO3, conforme mostrado nos sais MS. Avaliou-se a sobrevivência, tamanho e número de brotações do explante, e o valor da biomassa fresca. Após a aclimatização das mudas avaliou-se a sobrevivência, número de brotações, comprimento da parte aérea, número de raízes, formação da raquete, massa média da biomassa fresca, absorção de macronutrientes e alterações morfológicas das mudas. Os explantes inoculados em meio com fertilizantes nas concentrações de 0,0; 2,0 e 2,5 g L-¹ não se desenvolveram. A resposta dos explantes nas concentrações de 0,5 e 1,5 g L-1 do fertilizante foram iguais aos desenvolvidos em meio contendo KNO3, e na concentração de 1,0 g L-1, em todas as variáveis, as médias foram superiores em relação as do controle. Dessa forma, constatou-se a viabilidade do uso do fertilizante no cultivo in vitro da palma, o que propiciou a eliminação dos entraves burocráticos e redução no custo de 99,12% na compra do produto.

ABSTRACT: In micropropagation, potassium nitrate (KNO3), an ACS reagent grade chemical, used in the preparation of growing mediums is expensive and its procurement depends on bureaucratic procedures, as it is controlled by the Brazilian Army. This research to assessed the effect of replacing the ACS KNO3 for a commercially available fertilizer (KNO3- based) on the micropropagation of the prickly pear cactus (Opuntia stricta (Haw.) Haw. cv. Elephant Ear. Treatments used six different fertilizer concentrations (0, 0.5, 1, 1.5, 2 and 2.5 g L-1) and a control consisting of 1.9 g L-1 KNO3, as shown in the MS salts. The survival, size and number of sprouts and the value of fresh biomass were evaluated. After seedling acclimation, we assessed the survival, number of sprouts, length, and number of roots, racket formation, average fresh biomass mass, macronutrient absorption and morphological changes of the seedlings. Explants inoculated with fertilizers at concentrations of 0.0; 2.0 and 2.5 g L-¹ did not grow. The response of explants at concentrations of 0.5 and 1.5 g L-1 of the fertilizer were the same as those developed in a KNO3 medium, and at a concentration of 1.0 g L-1, in all variables, the means were higher than those of the control medium. Therefore, it showed the feasibility of using fertilizers in the in vitro cultivation of the prickly pear cactus, which may remove bureaucratic barriers and reduce product costs by 99.12%.

ABSTRACT Many students dislike Physical Education classes in Brazil because of content repetition, unsatisfactory curricular organization, among other factors. The purpose of this study is to assess the efficiency of a proposal for Physical Education classes, which includes contents of physical activities and the use of comic books application. This is a qualitative study, which comprises both bibliographic and field researches. Twelve classes were held to a group of eight graders from a public school in the city of Piracicaba/SP/Brazil. Questionnaires were used to evaluate the proposal for Physical Education classes. We found out that the use of comic books application is an effective teaching resource, as it reveals the students’ experiences, what they felt, and the mediation between their “rational” and “sensitive” knowledge about physical activities. There is evidence that our experience has provided participating students with: 1- Leisure education and health education, 2- Awakening to knowledge of the body culture of movement, 3- Access to some leisure contents, mainly those related to physical, sports and artistic ones, 4- The creation of comics by App Flaras.

RESUMEN A muchos estudiantes no les gustan las clases de Educación Física en Brasil debido a la repetición de contenidos, organización curricular insatisfactoria, entre otros factores. El propósito de este estudio es evaluar la eficiencia de una propuesta de clases de Educación Física, que incluye contenidos de actividades físicas y el uso de la aplicación de cómics. Se trata de un estudio cualitativo, que comprende tanto investigaciones bibliográficas como de campo. Se impartieron doce clases a un grupo de ocho alumnos de una escuela pública de la ciudad de Piracicaba / SP / Brasil. Se utilizaron cuestionarios para evaluar la propuesta de clases de Educación Física. Descubrimos que el uso de la aplicación de cómics es un recurso didáctico eficaz, ya que revela las experiencias de los estudiantes, lo que sentían y la mediación entre sus conocimientos "racionales" y "sensibles" sobre las actividades físicas. Existe evidencia de que nuestra experiencia ha proporcionado a los estudiantes: 1. Educación para el ocio participativa y educación para la salud, 2. Despertar al conocimiento de la cultura corporal del movimiento, 3. Acceso a algunos contenidos de ocio, principalmente los relacionados con los físicos, deportivos y artísticos, y 4. La creación de cómics por App Flaras.

Resumo Objetivos: descrever o perfil de mulheres acometidas por parto prematuro e os desfechos neonatais em maternidade de referência, na cidade de Fortaleza-CE, Brasil. Métodos: estudo do tipo documental, descritivo, retrospectivo, com abordagem quantitativa, realizadode janeiro a dezembro de 2017, com 253 prontuários de mulheres que tiveram parto prematuro em maternidade de referência. Resultados: média de idade de 28 anos, com prevalência de mulheres vivendo em união estável, ensino médio completo, sem trabalho formal. A média gestacional foi de três gravidezes, idade gestacional de 34 semanas e trêsdias e seis consultas de pré-natal, iniciando no primeiro trimestre. A maior intercorrência foi a pré-eclâmpsia. Relacionado aos dados neonatais, houve prevalência de recém-nascidos do sexo masculino, com média de 2,251 quilos e escore sete no Apgar do 1º minuto e oito, no Apgar do 5º minuto. Recémnascidos indo para alojamento conjunto após o parto e em ar ambiente, tendo passado, em média, 12,71 dias internados. Conclusões: no caso da pesquisa, conhecer o perfil dessa mulher e os desfechos do recém-nascido prematuro é útil para estimular as políticas públicas e diminuir as sequelas para mãe e bebê.

Abstract Objectives: to describe the profile of women affected with premature childbirth and neonatal outcomes at a referral maternity in the city of Fortaleza-CE, Brazil. Methods: descriptive and retrospective documentary type study, with a quantitative approach, carried out from January to December, 2017, with 253 medical records of women who had premature childbirth in a referral maternity. Results: the average age was 28, with the prevalence of women living in a stable union, graduated from high school and without formal work. The gestational mean average was three pregnancies, gestational age of 34 weeks and three days, and six prenatal consultations, starting in the first trimester. The major intercurrence was pre-eclampsia. In relation to the neonatal data, there was a prevalence of male newborns, with an average of 2.251 kg and a score of seven on the 1-minute Apgar and eight on the 5-minute Apgar. Newborns in going to a hospital accommodation after childbirth and in room air, spending an average of 12.71 days in the hospital. Conclusion: in this case of this research, knowing the woman’s profile and the outcomes in premature newborns is useful to encourage public policies and reduce the sequelae on mother and baby

ABSTRACT Sexually transmitted infections (STIs) represent a global health problem with variable prevalence depending on the geographical region and the type of population. Human papillomavirus (HPV) encompasses widespread virus types related to cervical carcinogenesis. The present study investigated the molecular prevalence of HPV and seven other important STIs in asymptomatic women working or studying at a Brazilian university. A secondary aim was to assess cytological abnormalities associated with HPV and other STIs coinfections. We recruited 210 women from a Brazilian university. HPV was detected using a single-round polymerase chain reaction (sPCR) followed by a viral genotyping by restriction fragment length polymorphism (RFLP-PCR). The presence of seven STIs: Chlamydia trachomatis, Neisseria gonorrhoeae, Treponema pallidum, Trichomonas vaginalis, Mycoplasma genitalium, herpes simplex virus (HSV)-1 and HSV-2 was detected by multiplex PCR (M-PCR). Furthermore, cytological findings and epidemiological characteristics were evaluated.The mean age of the participants was 27.1 years old. HPV prevalence was 33.8%, and HPV16 was the most frequently detected papillomavirus genotype. Moreover, multiple HPV infections were common (42.2%). We detected at least one STI agent in 11.4% of the tested women, most frequently C. trachomatis (6.7%). Among HPV-positive women, 14.1% were coinfected with other STI agents. Cytological abnormalities were observed in 9.5% of smears, and HPV-DNA, high-risk HPV (HR-HPV), HPV16 and HPV multiple infections were associated with abnormal cytological findings. There was a high prevalence of HPV, and C. trachomatis was the most prevalent STI agent, with low rates of cytological abnormalities. These findings highlight the need of timely STI diagnosis in young asymptomatic women and of a public policy design for STI prevention.

SUMMARY: Mesenchymal cells (MCs) exhibit great regenerative potential due to their intrinsic properties and ability to restore tissue function, either directly through transdifferentiation or indirectly through paracrine effects. This study aimed to evaluate morphometric and phenotypic changes in MCs grown with facial nerve-conditioned medium in the presence or absence of fibroblast growth factor 2 (FGF-2). For quantitative phenotypic analysis, the expression of GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200 was analyzed by immunocytochemistry. Cells cultured with facial nerve-conditioned medium in the presence of FGF-2 expressed GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200. On average, the area and perimeter of GFAP-positive cells were higher in the group cultured with facial nerve-conditioned medium compared to the group cultured with conditioned medium and FGF-2 (p=0.0001). This study demonstrated the plasticity of MCs for neuronal and glial lineages and opens up new research perspectives in cell therapy and trans.differentiation.

RESUMEN: Las células mesenquimales (CM) exhiben un gran potencial regenerativo debido a sus propiedades intrínsecas y la capacidad de restaurar la función del tejido, ya sea directamente, a través de la transdiferenciación, o indirectamente, a través de efectos parácrinos. Este estudio tuvo como objetivo evaluar los cambios morfométricos y fenotípicos en CM cultivadas con medio condicionado por nervio facial en presencia o ausencia de factor de crecimiento de fibroblastos 2 (FGF-2). Para el análisis fenotípico cuantitativo, se analizó la expresión de GFAP, OX-42, MAP-2, β-tubulina III, NeuN y NF-200 mediante inmunocitoquímica. Las células cultivadas con medio condicionado por el nervio facial en presencia de FGF-2 expresaban GFAP, OX-42, MAP-2, β-tubulina III, NeuN y NF-200. En promedio, el área y el perímetro de las células positivas para GFAP fueron mayores en el grupo cultivado con medio condicionado por el nervio facial en comparación con el grupo cultivado con medio acondicionado y FGF-2 (p = 0,0001). Este estudio demostró la plasticidad de CM para linajes neuronales y gliales y abre nuevas perspectivas de investigación en terapia celular y transdiferenciación.

Resumo Fundamentos: A hipercolesterolemia familiar (HF) é uma doença autossômica dominante, caracterizada por altos níveis plasmáticos do colesterol da lipoproteína de baixa densidade (LDL-C) e pelo alto risco de desenvolvimento prematuro de doenças cardiovasculares. Objetivo: Avaliar características clínicas e antropométricas de pacientes com fenótipo para hipercolesterolemia familiar (HF), com ou sem diagnóstico genético de HF. Métodos: Quarenta e cinco pacientes com LDL-C > 190 mg/dL foram genotipados para seis genes relacionados com a HF: LDLR, APOB, PCSK9, LDLRAP1, LIPA e APOE. Pacientes que apresentaram resultado positivo para qualquer uma das mutações foram diagnosticados com HF por confirmação genética. O fenótipo para HF foi classificado pelo critério da Dutch Lipid Clinic Network. Resultados: Comparando os pacientes com a HF geneticamente confirmada com aqueles sem a confirmação, os primeiros apresentaram maior pontuação do escore para HF, uma maior frequência de xantelasma e maiores níveis de LDL-C e apo B. Houve correlações significativas entre o LDL-C e a pontuação do escore para HF (R = 0,382, p = 0,037) e entre LDL-C e gordura corporal (R = 0,461, p = 0,01). Os pacientes com mutações, no entanto, não apresentaram qualquer correlação entre o LDL-C e outras variáveis, enquanto aqueles sem mutação apresentaram correlação entre o LDL-C e a pontuação do escore. Conclusão: O LDL-C correlacionou-se com a pontuação do escore e com a gordura corporal, tanto na população total de pacientes quanto nos pacientes sem a confirmação genética de HF. Naqueles com HF geneticamente confirmada, não houve correlação entre o LDL-C e outras variáveis clínicas ou bioquímicas dos pacientes.

Abstract Background: Familial hypercholesterolemia (FH) is a common autosomal dominant disorder, characterized by a high level of low-density lipoprotein cholesterol (LDL-C) and a high risk of premature cardiovascular disease. Objective: To evaluate clinical and anthropometric characteristics of patients with the familiar hypercholesterolemia (FH) phenotype, with or without genetic confirmation of FH. Methods: Forty-five patients with LDL-C > 190 mg/dl were genotyped for six FH-related genes: LDLR, APOB, PCSK9, LDLRAP1, LIPA and APOE. Patients who tested positive for any of these mutations were considered to have genetically confirmed FH. The FH phenotype was classified according to the Dutch Lipid Clinic Network criteria. Results: Comparing patients with genetically confirmed FH to those without it, the former had a higher clinical score for FH, more often had xanthelasma and had higher LDL-C and apo B levels. There were significant correlations between LDL-C and the clinical point score for FH (R = 0.382, p = 0.037) and between LDL-C and body fat (R = 0.461, p = 0.01). However, patients with mutations did not have any correlation between LDL-C and other variables, while for those without a mutation, there was a correlation between LDL-C and the clinical point score. Conclusions: LDL-C correlated with the clinical point score and with body fat, both in the overall patient population and in patients without the genetic confirmation of FH. In those with genetically confirmed FH, there were no correlations between LDL-C and other clinical or biochemical variables in patients.

RESUMO O objetivo deste artigo foi quantificar e analisar as alterações ocorridas, entre os anos 1998 e 2012, no valor bruto da produção da pimenta-do-reino no Estado do Pará, bem como as mudanças nos fatores de sua variação: área colhida, produtividade e preço. Para tal, foram utilizadas a taxa geométrica de crescimento, o coeficiente de variação e o modelo “shift-share”. De maneira geral, o valor bruto da produção foi crescente no período estudado, passando por um período inicial de retração, com recuperação do crescimento a partir de 2006. A área colhida e o preço aumentaram no período de 1998 a 2012, enquanto a produtividade da cultura diminuiu. O preço apresentou maior oscilação dentre os fatores estudados e teve o maior impacto no valor bruto de produção. A produtividade se mostrou a mais estável. Aumentos na área colhida e principalmente na produtividade foram considerados de interesse para que a cultura da pimenta-do-reino cresça em valor no mercado.

ABSTRACT The aim of this paper was to quantify and analyze the changes occurred, between the years 1998 to 2012, in the gross value of production of the black pepper in the State of Pará, as well as the changes in the factors of its variation: harvested area, crop yield and price. For the analysis, it was used the geometric rate of growth, the coefficient of variation and the “shift-share” model applied to the culture's data. Overall, the gross value of production increased in the studied period, passing through an initial period of decline, with recovery of growth in 2006. The harvested area and the selling price increased between the period of 1998 to 2012, while the crop yield decreased. The price showed greater oscillation among the studied factors and had the highest impact in the gross value of production. The productivity proved to be more stable. Increases in harvested area and especially in productivity were considered of interest so the culture of black pepper can grow in market value.

RESUMO Objetivo: estimar a prevalência da probabilidade de transtorno mental comum entre indivíduos que abusam de álcool e outras drogas. Método: estudo transversal. A amostra constituiu-se de 234 indivíduos em tratamento e reabilitação de dependência química em clínicas e em um Centro de Atenção Psicossocial na região sudeste do Estado de Goiás. Foram aplicados instrumentos sobre perfil sociodemográfico, consumo de drogas lícitas e/ou ilícitas, e o questionário que rastreia sofrimento mental comum. Resultados: a variável desfecho foi a probabilidade de transtorno mental comum, com 37,2% do total da amostra. Houve associação da probabilidade para transtorno mental comum com sexo feminino (odds ratio=4,06; IC95% 1,56-10,56), idade ≤25 anos (odds ratio=3,00; IC95% 1,41-6,38) e uso de maconha na vida (odds ratio=2,83; IC95% 1,38-5,78). Conclusão: o estudo revelou a associação entre uso e abuso de drogas com o risco para transtorno mental comum.

ABSTRACT Objective: to estimate the prevalence of the probability of common mental disorders among abusers of alcohol and other drugs. Method: a cross-sectional study. The sample was made up of 234 individuals undergoing treatment and rehabilitation for chemical dependence in private clinics and in a Psychosocial Care Center in the Southeast area of the state of Goiás, Brazil. Instruments on sociodemographic profile, use of licit and/or illicit drugs, and a questionnaire that tracks common mental suffering were applied. Results: the outcome variable was the probability of common mental disorder, with 37.2% of the total of the sample. Association between the probability of common mental disorders and the female gender (odds ratio=4.06; 95% CI 1.56-10.56), age ≤25 years old (odds ratio=3.00; 95% CCI 1.41-6.38), and use of marijuana in life (odds ratio=2.83; 95% CI 1.38-5.78) were found. Conclusion: the study showed an association between the use and abuse of drugs and risk for common mental disorders.

RESUMEN Objetivo: estimar la prevalencia de probabilidad de trastorno mental común entre sujetos que abusan de alcohol y otras substancias. Metodo: estudio trasversal. La muestra se constituyó de 234 sujetos en tratamiento y rehabilitación por la dependencia química en clínicas y en un Centro de Atención Psicosocial en la región sudeste del Estado de Goiás, en Brasil. Fueron aplicados instrumentos sobre el perfil sociodemográfico, el consumo de drogas lícitas y/o ilícitas y el cuestionario que rastrea el sufrimiento mental común. Resultados: la variable de resultado fue la probabilidad de trastorno mental común, con 37,2% del total de la muestra. Hubo una asociación de la probabilidad para Trastorno Mental Común, con el sexo femenino (odds ratio=4,06; IC95% 1,56-10,56), la edad ≤25 años (odds ratio=3,00; IC95% 1,41-6,38) y el uso de marihuana (odds ratio=2,83; IC95% 1,38-5,78). Conclusión: el estudio reveló una asociación entre el uso y abuso de substancias con el riesgo para trastorno mental común.

ABSTRACT Objective: to estimate the prevalence of the probability of common mental disorders among abusers of alcohol and other drugs. Method: a cross-sectional study. The sample was made up of 234 individuals undergoing treatment and rehabilitation for chemical dependence in private clinics and in a Psychosocial Care Center in the Southeast area of the state of Goiás, Brazil. Instruments on sociodemographic profile, use of licit and/or illicit drugs, and a questionnaire that tracks common mental suffering were applied. Results: the outcome variable was the probability of common mental disorder, with 37.2% of the total of the sample. Association between the probability of common mental disorders and the female gender (odds ratio=4.06; 95% CI 1.56-10.56), age ≤25 years old (odds ratio=3.00; 95% CCI 1.41-6.38), and use of marijuana in life (odds ratio=2.83; 95% CI 1.38-5.78) were found. Conclusion: the study showed an association between the use and abuse of drugs and risk for common mental disorders.

RESUMEN Objetivo: estimar la prevalencia de probabilidad de trastorno mental común entre sujetos que abusan de alcohol y otras substancias. Metodo: estudio trasversal. La muestra se constituyó de 234 sujetos en tratamiento y rehabilitación por la dependencia química en clínicas y en un Centro de Atención Psicosocial en la región sudeste del Estado de Goiás, en Brasil. Fueron aplicados instrumentos sobre el perfil sociodemográfico, el consumo de drogas lícitas y/o ilícitas y el cuestionario que rastrea el sufrimiento mental común. Resultados: la variable de resultado fue la probabilidad de trastorno mental común, con 37,2% del total de la muestra. Hubo una asociación de la probabilidad para Trastorno Mental Común, con el sexo femenino (odds ratio=4,06; IC95% 1,56-10,56), la edad ≤25 años (odds ratio=3,00; IC95% 1,41-6,38) y el uso de marihuana (odds ratio=2,83; IC95% 1,38-5,78). Conclusión: el estudio reveló una asociación entre el uso y abuso de substancias con el riesgo para trastorno mental común.

RESUMO Objetivo: estimar a prevalência da probabilidade de transtorno mental comum entre indivíduos que abusam de álcool e outras drogas. Método: estudo transversal. A amostra constituiu-se de 234 indivíduos em tratamento e reabilitação de dependência química em clínicas e em um Centro de Atenção Psicossocial na região sudeste do Estado de Goiás. Foram aplicados instrumentos sobre perfil sociodemográfico, consumo de drogas lícitas e/ou ilícitas, e o questionário que rastreia sofrimento mental comum. Resultados: a variável desfecho foi a probabilidade de transtorno mental comum, com 37,2% do total da amostra. Houve associação da probabilidade para transtorno mental comum com sexo feminino (odds ratio=4,06; IC95% 1,56-10,56), idade ≤25 anos (odds ratio=3,00; IC95% 1,41-6,38) e uso de maconha na vida (odds ratio=2,83; IC95% 1,38-5,78). Conclusão: o estudo revelou a associação entre uso e abuso de drogas com o risco para transtorno mental comum.

A descriptive study based on data obtained from a toxicological information and assistance center, from 2007 to 2011. This study aimed to characterize hospitalizations of victims of accidents with venomous animals, in order to support the development of preventive and assistance measures. Data were tabulated using the Epi Info 6.04d® program; and the results were presented in tables and figure. 344 hospitalizations were found, with predominance of male patients (58.1%), from 20 to 59 years (56.8%), mostly in the summer (39.0%) spring (27.0%), for snakebites (35.2%). The hospital stay ranged from one to 23 days, with 39.0% of patients hospitalized for two or more days, with two deadly accidents with bees. The profile of the inpatients showed a higher number of cases in the economically active population and in males, the percentage of hospitalizations per animal aggressor differed from morbidity data, giving greater severity of accidents by snakes and bees.

Estudio descriptivo, basado en datos del Centro de información y asistencia toxicológica, de 2007 a 2011. El objetivo fue caracterizar los ingresos de víctimas de envenenamientos, con el fin de aportar al desarrollo de medidas de prevención y atención. Los datos fueron tabulados utilizando Epi Info 6.04d ®, y los resultados se presentan en tablas y gráficos. Se encontraron 344 admisiones, con predominio de pacientes del sexo masculino (58,1%), 20 a 59 años (56,8%), mayoría en verano (39,0%), primavera (27,0%), mordeduras de serpiente (35,2%). La estancia hospitalaria varió de uno a 23 días, con 39,0% hospitalizados durante dos o más días, dos muertos tras accidente con abejas. El perfil de los pacientes presentó mayor número de casos en la población económicamente activa, del sexo masculino, el porcentaje de los ingresos por animal agresor, difieren de datos de morbilidad, dando mayor severidad a los accidentes por serpientes y abejas.

Estudo descritivo, a partir de dados de um centro de informação e assistência toxicológica, do período de 2007 a 2011. Objetivou-se caracterizar internações de vítimas de acidentes por animais peçonhentos, com vistas a fornecer subsídios à elaboração de medidas preventivas e assistenciais. Os dados foram tabulados pelo programa Epi Info 6.04d®, sendo os resultados apresentados em tabelas e gráficos. Encontrou-se 344 internações, com predomínio de pacientes do sexo masculino (58,1%), com 20 a 59 anos (56,8%); a maioria no verão (39,0%) e na primavera (27,0%), por acidentes ofídicos (35,2%). A internação variou de um a 23 dias, com 39,0% internados por dois ou mais dias, ocorrendo dois óbitos, após acidente com abelhas. O perfil dos internados apontou maior número de casos na população economicamente ativa, no sexo masculino e, o percentual das internações, por animal agressor, diferiu dos dados de morbidade, conferindo maior gravidade aos acidentes por serpentes e abelhas.

Rangelia vitalii é um protozoário que causa doença em cães, sendo a anemia o achado laboratorial mais frequente. No entanto, existem poucos estudos sobre as alterações bioquímicas em cães infectados com o protozoário. Assim, este estudo tem como objetivo investigar as alterações bioquímicas de cães experimentalmente infectados com R. vitalii na fase aguda da infecção. Para o estudo, foram utilizados 12 cães fêmeas (com idade entre 6 a 12 meses e peso entre 4 a 7 kg), divididos em dois grupos. O grupo A (n = 5) foi composto de animais saudáveis e o grupo B (n = 7) de animais infectados. Amostras de sangue foram coletadas nos dias zero, dez, vinte e trinta PI, utilizando tubos sem anticoagulante para obtenção de soro e análise dos parâmetros bioquímicos. Foi observado um aumento na alanino aminotransferase (ALT) no dia 20 PI (P < 0,05) e aumento na creatinoquinase (CK) e aspartato aminotransferase (AST) em todo o período experimental (P < 0,05). Não foram observadas alterações séricas na gama-glutamiltransferase, uréia e creatinina. Portanto, é possível concluir que a infecção experimental por R. vitalii causa alterações no perfil bioquímico, com aumento na ALT, CK e AST.

Rangelia vitalii is a protozoon that causes diseases in dogs, and anemia is the most common laboratory finding. However, few studies on the biochemical changes in dogs infected with this protozoon exist. Thus, this study aimed to investigate the biochemical changes in dogs experimentally infected with R. vitalii, during the acute phase of the infection. For this study, 12 female dogs (aged 6-12 months and weighing between 4 and 7 kg) were used, divided in two groups. Group A was composed of healthy dogs (n = 5); and group B consisted of infected animals (n = 7). Blood samples were collected on days 0, 10, 20 and 30 after infection, using tubes without anticoagulant to obtain serum and analyze the biochemical parameters. An increase in alanine aminotransferase (ALT) on day 20 (P < 0.05) was observed. Also, increased creatine kinase (CK) and aspartate aminotransferase (AST) levels were observed throughout the experimental period (P < 0.05). No changes in the serum gamma-glutamyltransferase, urea and creatinine levels were observed. Thus, is possible to conclude that experimental infection with R. vitalii in dogs causes changes to the biochemical profile, with increased ALT, AST and CK enzyme levels.

AIM: To investigate, using the registry of medical records, how childcare is performed by the teams of the Family Health Strategy, working at a Basic Health Unit in the city of Maringá, Paraná. METHOD: Data were collected in January 2011, starting with the consultation of medical records of 181 children born between July 2008 and January 2010, in which relevant data were observed on child monitoring during the first year of life. RESULTS: A nurse was responsible for 51.9% of the childcare consultations. It was further observed that the proportion of unfilled charts in various fields was high. DISCUSSION: The number of childcare attendances made by nurses demonstrates their importance in monitoring the health of children. FINA CONSIDERATIONS: It is believed that the implementation of a standard form to record the childcare consultations should minimize the underreporting of the follow-up activities to children for facilitating and systematizing the annotation.

The objective of this study was to evaluate the inclusion of yeast extract as a partial replacemer of blood plasma in piglet diets and its effect on the performance and intestinal morphometry of pigs weaned at 21 days of age. One hundred and twenty animals were randomized into blocks, with five diets (4.0% blood plasma; 2.0% blood plasma with 0.0; 1.0; 2.0 or 3.0% yeast extract), six replicates and four pigs per experimental unit. At 35 days of age, one pig per experimental unit was slaughtered in order to evaluate duodenal and jejunal morphometry. The levels of yeast extract had a quadratic impact on daily feed intake and final weight, which increased up to the estimated level of 1.91%. Daily weight gain and feed conversion rate were not affected. A comparison between diets containing different levels of yeast extract and the basal diet showed that the diets containing 2.0% and 1.0% yeast extract provided the highest final weight and the worst feed conversion rate, respectively. Duodenal villus height and crypt depth were quadratically affected by yeast extract levels and the best results were achieved with the estimated levels of 1.64 and 1.16%, respectively. The levels of yeast extract provided a linear increase in the duodenal villus:crypt ratio, but did not change the morphometric variables of the jejunum. No difference was found in the morphometric variables of the duodenum and jejunum when diets containing different levels of yeast extract were compared with the basal diet. The results suggest that the inclusion of 1.91% yeast extract allows for a partial replacement for plasma in the diet, decreasing plasma inclusion from 4.0 to 2.0%.

With the objective to evaluate levels of available phosphorus (aP) in diets with or without ractopamine, 112 gilts with high genetic potential for meat deposition with initial weigh of 94.0±2.28 kg were allotted in a completely randomized block design, in a 4 × 2 factorial arrangement (0.109, 0.209, 0.309, and 0.409% aP and 0 or 5 ppm of ractopamine), with eight treatments, seven replicates, and two gilts per experimental unit. There were no interactions between aP levels and ractopamine. The levels of available phosphorus influenced both daily weight gain and daily phosphorus intake, which increased linearly. Feed conversion improved in a quadratic way up to the estimated level of 0.330% aP maximum response. Daily weight gain increased and feed conversion improved by adding ractopamine to the diets. Serum alkaline phosphatase activity decreased linearly by increasing the levels of available phosphorus. Bone strength, ash, calcium, and phosphorus contents in the bones also increased quadratically with maximum responses at 0.320, 0.270, 0.277, and 0.295% aP, respectively. The available phosphorus level of 0.330%, corresponding to a daily intake of 8.67 g of aP is suitable for 94 to 125 kg gilts selected for meat deposition fed diets with or without ractopamine.

Este estudo foi realizado com o objetivo de avaliar níveis de zeólita em dietas para suínos e seus efeitos no desempenho, nas características de carcaça e nos parâmetros sanguíneos e histopatológicos do parênquima hepático desses animais nas fases de crescimento e terminação. Foram utilizados 84 suínos machos castrados, híbridos comerciais de alto potencial genético para deposição de carne, com peso inicial de 30,34±1,14 kg, distribuídos em delineamento experimental de blocos ao acaso, com sete níveis de adsorvente (0,0-0,0; 1,0-0,5; 1,0-1,0; 2,0-1,0; 2,0-2,0; 3,0-1,5; 3,0-3,0, respectivamente, nas fases de crescimento e terminação), com seis repetições e dois animais por baia (unidade experimental). Utilizou-se o sorgo como fonte de contaminação natural por micotoxinas. Os níveis de adsorvente das dietas experimentais foram obtidos a partir da inclusão de zeólita em substituição à areia lavada das dietas sem adsorvente. Não houve efeito dos níveis de zeólita nas dietas sobre o consumo diário de ração, o ganho de peso diário, a conversão alimentar e as características carcaça avaliadas. Os níveis de zeólita nas dietas também não influenciaram os parâmetros sanguíneos e histopatológicos nem os teores ósseos de cálcio e fósforo dos suínos. Devido ao bom índice de desempenho e às características de carcaça, bem como à normalidade dos outros parâmetros estudados dos animais que receberam dieta sem adsorvente, pode-se inferir que as dietas continham baixo grau de contaminação por micotoxinas. Os níveis de zeólita avaliados não influenciam o desempenho, as características de carcaça nem os parâmetros sanguíneos e histopatológicos do parênquima hepático de suínos nas fases de crescimento e terminação recebendo dietas com baixo nível de contaminação por micotoxinas.

This study was conducted to evaluate levels of inclusion of zeolite in diets for pigs on performance, carcass characteristics, blood and liver histopathological parameters of these animals in their growing-finishing phases. Eighty-four barrows from commercial hybrids of high genetic potential for meat deposition, with initial weight of 30.34±1.14 kg were distributed in randomized blocks design, with seven levels of adsorbent (0.0-0.0; 1.0-0.5; 1.0-1.0; 2.0-1.0; 2.0-2.0; 3.0-1.5; 3.0-3.0, respectively, on growing and finishing phases), six replicates with two animals per pen, which was considered the experimental unit. Sorghum was used as naturally contaminated source of mycotoxins. The different levels of adsorbent in the diets were obtained by the supplementation of zeolite in the basal diet. The inclusion of zeolite in the diet did not affect the daily feed intake, daily weight gain, feed conversion or the carcass characteristics of the pigs. In the same way blood parameters, liver histopathological examination and the bone concentration neither calcium nor phosphorus were affected by the different levels of zeolita. Due to the good performance and carcass characteristics, as well as the normality of the other parameters of the animals fed diets without adsorbents analyzed, it can be inferred that the diets had low levels of mycotoxin contamination. The levels of zeolite do not affect the performance, carcass characteristics, blood parameters or liver histopathological examination in growing-finishing pigs fed diets with low level of contamination by mycotoxin.