Resumen El artículo propone reflexiones sobre las relaciones interdisciplinarias que consideran el espacio, la emoción y la memoria. Explora aspectos de la influencia cultural y de responsabilidad social del Diseño en la actualidad, las posibilidades de diálogo con otras áreas del conocimiento y de la capacidad de tejer relaciones entre saberes. Se pone en escena una experiencia proyectual creativa en el campo del diseño social, cuyos principios teóricos y prácticas metodológicas fundamentaron un ensayo fotográfico. La experiencia única resultó en el libro “Viejos recuerdos, memorias de vida”, inspirado en los informes afectivos de catorce personas ancianas alojadas en la Vila Vicentina, un espacio de «un evento solidario”. Aunque desplazados de sus orígenes, el sentido de la realidad no se pierde y el nuevo espacio se convierte en definitivo, para que las reminiscencias del pasado convivan con el presente y propongan una mirada al futuro.

Abstract The article proposes reflections on interdisciplinary relationships that consider space, emotion and memory. It explores aspects of design’s cultural influence and social responsibility, possibilities for dialogue with other areas of knowledge, and the ability to weave relationships between knowledge. A creative project experience in the field of Social Design is set in motion, whose theoretical principles and methodological practices underlie a photographic essay. The unique experience resulted in the book “Old memories, memories of life”, inspired by the affective reports of fourteen elderly people housed in Vila Vicentina, a space of ‘a solidarity happening’. Although displaced from their origins, the sense of reality is not lost and the new space becomes definitive for reminiscences of the past to live with the present and propose a look to the future.

Resumo O artigo propõe reflexões acerca das relações interdisciplinares que consideram espaço, emoção e memória. Explora aspectos da influência cultural e responsabilidade social do Design na atualidade, possibilidades de diálogo com outras áreas do conhecimento e capacidade de tecer relações entre saberes. Coloca-se em cena uma experiência projetual criativa na área do Design Social, cujos princípios teóricos e práticas metodológicas fundamentaram um ensaio fotográfico. A vivência singular resultou no livro “Velhas lembranças, memórias de vida”, inspirado nos relatos afetivos de quatorze idosos abrigados na Vila Vicentina, espaço de ‘um acontecer solidário’. Embora deslocados de suas origens, a sensação de realidade não se perde e o novo espaço torna-se definitivo para que reminiscências do passado convivam com o presente e proponham um olhar para o futuro.

ABSTRACT The control of dyslipidemia by using herbal products is an important subject for studies. In this study, we evaluated the effects of dry Passiflora incarnata L. extract over dyslipidemia, left ventricular hypertrophy, and hepatic oxidative stress of LDL receptor knockout mice (LDLr-/-). Forty 4-month old male LDLr-/- mice were distributed into four groups: Group standard diet; Group standard diet and 200 mg/kg of body weight of Passiflora incarnata L. leaf dry extract; Group high-fat diet; Group high-fat diet and 200 mg/kg of body weight of Passiflora incarnata L. leaf dry extract. After 30 days, Passiflora incarnata L. dry extract reduced the effects of the high-fat diet, with a decrease of total cholesterol, triglycerides, and increase of high-density lipoprotein (HDL), as well as a reduction of C-reactive protein, alkaline phosphatase and insulin. There was no effect on glucose, Homa index and enzymes aspartate aminotransferase and alanine aminotransferase. However, the prevention of left ventricular hypertrophy occurred, as well as lipid peroxidation and the production of carbonyl proteins, which are both oxidative stress markers. In conclusion, Passiflora incarnata L. dry extract acts in the prevention of dyslipidemia, consequently, hindering the occurrence of hepatic oxidative stress and the development of left ventricular hypertrophy by the increase of serum HDL, in mice that had the effects of a high-fat diet.

Bacteriocins are antibacterial, proteinaceous substances that mediate microbial dynamics. Bacteriocin production is a highly disseminated property among all major lineages of bacteria, including Shigella. In this paper, we addressed the purification and characterisation of a bacteriocin produced by a Shigella sonnei strain (SS9) isolated from a child with acute diarrhoea. The substance was purified through ammonium-sulphate precipitation and sequential steps of chromatography. The intracellular fraction obtained at 75% ammonium sulphate maintained activity following exposure to pH values from 1-11 and storage at -80ºC for more than two years and was inactivated by high temperatures and proteases. The molecular mass of the purified bacteriocin was determined by mass spectrometry to be 18.56 kDa. The N-terminal sequence of the bacteriocin did not match any other antibacterial proteins described. A putative new bacteriocin produced by S. sonnei has been detected. This bacteriocin may represent a newly described protein or a previously described protein with a newly detected function. Considering that SS9 expresses antagonism against other diarrhoeagenic bacteria, the bacteriocin may contribute to S. sonnei virulence and is potentially applicable to either preventing or controlling diarrhoeal disease.