Abstract Background: The use of doxorubicin in chemotherapy has been associated with cardiotoxicity and heart failure. Physical exercise produces favorable morphofunctional adaptations in the cardiovascular system and may reverse cardiac dysfunction in patients undergoing chemotherapy. Objective: To assess the effects of physical training on myocardial structure, cardiac function, and exercise tolerance in Wistar rats initiated after the onset of cardiotoxicity-induced cardiotoxicity. Methods: This study investigated 30 adult male Wistar rats randomly divided into four groups: control (C), exercise (EX), doxorubicin (DX), and doxorubicin and exercise (DXEX). The DX and DXEX groups received six doses of doxorubincin from 1.25 mg/kg body weight up to a cumulative dose of 7.5 mg/kg. Injections were administered intraperitoneally three times a week for two weeks; after this stage, the EX and DXEX groups started physical training (swimming) sessions three times a week with a load of 5% of their body weight. Echocardiography and exercise tolerance tests were performed. Generalized linear models were used in statistical analysis, and a p<0.05 was set as statistically significant. Results: Left ventricular shortening fraction and ejection fraction were reduced in the DX group compared to C, EX, and DXEX. The DXEX group showed greater tolerance to effort when compared to the DX and C groups. Conclusion: Physical training, initiated after the onset of doxorubicin-induced cardiotoxicity, improved cardiac function and exercise tolerance in rats.

Abstract The incidence of aflatoxins and ochratoxin A in spices purchased from São Paulo State, Brazil was investigated. A total of 180 black pepper (Piper nigrum L.), colorífico (mixture of cornmeal or cassava flour with powdered annatto, Bixa orellana) ginger (Zingiber officinale Roscoe), nutmeg (Myristica fragrans), paprika (Capsicum annuum L.), and turmeric (Curcuma longa) were analyzed with a modified methods by using immunoaffinity column for clean-up and liquid chromatography/fluorescence detector for separation and quantification. Analytical methods were optimized for each spice, focusing mainly on the extraction step. OTA recoveries ranged from 65-102%. AFs recoveries were >70% except for AFG2. The average levels of AFs and OTA in black pepper, colorífico and turmeric samples were less than 2 ng/g. Twenty-five ginger samples (100%) contained OTA 0.10 - 7.10 ng/g and 21 samples (84%) contained AFs 0.10 - 9.55 ng/g. Twenty-nine nutmeg samples (100%) contained OTA 0.92 - 65.49 ng/g and AFs 2.71 - 48.67 ng/g. Thirty paprika samples (100%) contained OTA, 0.75 - 147.18 ng/g and twenty-two samples (73%) contained AFs, 0.11 - 14.92 ng/g. AFs and OTA in nutmeg and paprika could represent a food safety issue in Brazil.

RESUMO A área de Comportamento Motor (CoM) investiga os processos neuropsicológicos da organização da resposta motora em termos de aprendizagem, controle e desenvolvimento motor, de onde emergiram. Este estudo teve como objetivo descrever a produção intelectual da área de Comportamento Motor (CoM), e as especificidades das suas subáreas de investigação denominadas Aprendizagem Motora (AM), Controle Motor (CM) e Desenvolvimento Motor (DM) produzidos pelos programas de Pós-graduação em Educação Física no ano de 2018. A produção intelectual nas subáreas do CoM foi organizada, por estrato, tipo de tarefa e aderência com os periódicos da área 21 da CAPES. Foram encontrados 53 artigos publicados por 51 pesquisadores. A distribuição dos artigos por subáreas foi representada por 30,18% AM, 54,72% CM e 15,10% DM. A subárea CM apresentou 86% de publicação dos artigos nos estratos superiores, a subárea AM com 37%, seguida da subárea DM com 13%. A média das três subáreas resultou em 83,02% de estudos com tarefa simples construída em laboratório e somente 16,98% com tarefa complexa. O maior número de publicações com baixa aderência aliado ao maior número de pesquisas com tarefas simples parece demonstrar a dificuldade dos pesquisadores de aproximar os conhecimentos produzidos na área de CoM com a formação de um corpo de conhecimento específico da Educação Física.

ABSTRACT Motor Behavior (MB) is the study of neuropsychological processes with regard to the motor response organization in terms of learning, control, and motor development. Three research sub-areas emerged from MB, that is, Motor Learning (ML), Motor Control (MC), and Motor Development (MD). The present study aimed at describing the intellectual production related to ML, MC and MD sub-areas in 2018, in addition to identifying the current profile of the MB area. The intellectual production of the MB sub-areas was organized according to stratum, type of task, and adherence to the journals of area 21 (CAPES). Fifty three articles on MB, published by 51 researchers, were found. All these studies were produced by the Graduate Programs of Physical Education in 2018. The distribution of the articles in sub-areas was represented as follows: 30.18% ML, 54.72% MC, and 15.10% MD. The MC sub-area had 86% of its articles published in the upper strata; the ML sub-area had 37%, followed by the MD sub-area with 13%. The average of the three sub-areas resulted in 83.02% of studies with simple tasks and only 16.98% with complex tasks. The greater number of publications with low adherence combined with the greater number of investigations with simple tasks seems to show the researchers' difficulty in approaching the knowledge produced in the MB area for undergraduate Physical Education students.

RESUMO Introdução: As manifestações neurológicas causadas pela COVID-19 ainda não estão completamente elucidadas. O comprometimento neurológico pode decorrer de um efeito viral direto em neurônios ou em células gliais, a efeito imunomediado em resposta à infecção viral, ou de um efeito secundário a estados de hipercoagulabilidade e danos endoteliais, assim como decorrente de complicações sistêmicas graves relacionadas a cuidados intensivos prolongados na unidade de terapia intensiva. Objetivo: Descrever dois pacientes com recuperação tardia do nível de consciência após a retirada da sedação associados à infecção grave pelo SARS-CoV-2, que apresentaram lesões multifocais de substância branca, compatíveis com o diagnóstico de encefalomielite disseminada aguda. Métodos: Estudo observacional, com relato de dois casos de infecção grave pela COVID-19, em dois hospitais terciários na cidade de São Paulo, Brasil. Resultados: Os pacientes foram submetidos à investigação sistêmica e neurológica para avaliação de estado alterado de consciência após retirada de sedação. A ressonância magnética de crânio evidenciou lesões multifocais no centro semioval, sugestivos de processo inflamatório desmielinizante. Análise liquórica evidenciou PCR negativo para SARS-CoV-2 em ambos os casos. Conclusão: Lesões multifocais de substância branca podem ocorrer em pacientes com COVID-19, possivelmente associadas a estados alterados de consciência. Estudos adicionais são necessários para determinar o processo fisiopatológico da infecção viral e dos estados inflamatórios e imunomediados na gênese das manifestações neurológicas causadas pela COVID-19.

ABSTRACT Background: Neurological manifestations of COVID-19 are still incompletely understood. Neurological manifestations may be due to direct viral effect on neurons and glial cells, to an immune-mediated response to the virus, or to a hypercoagulable state and associated endothelial damage, as well as to severe systemic disease with prolonged intensive care unit stay. Objective: To describe two patients with severe SARS-CoV-2 infection and delayed recovery of consciousness after sedation withdrawal, in whom MRI disclosed multifocal white matter brain lesions, compatible with the diagnosis of acute disseminated encephalomyelitis. Methods: Observational report of two cases of severe COVID-19 infection in patients from two tertiary hospitals in São Paulo, Brazil. Results: These patients underwent neurologic and systemic evaluation for delayed awakening after sedation withdrawal. MRI displayed multifocal centrum semiovale lesions, suggestive of demyelinating inflammation. Cerebrospinal fluid (CSF) polymerase chain reaction (PCR) for SARS-CoV-2 was negative in both cases. Conclusion: A recurrent pattern of multifocal white matter lesions can occur in COVID-19 patients, possibly associated with delayed awakening. Additional studies are necessary to elucidate the role of the viral infection and of inflammatory and immune-mediated associated changes in neurological manifestations of COVID-19.

Resumo A vitamina D (1,25-dihidroxicolecalciferol) é um pró-hormônio que tem despertado a atenção de pesquisadores após estudos demonstrarem que seus efeitos não estão restritos ao metabolismo ósseo. Assim, a presente revisão sintetiza os achados mais recentes e discute a utilidade da prescrição de vitamina D e seus análogos no tratamento e prevenção de afecções cardiovasculares e disfunção endotelial. Este trabalho consiste em uma revisão narrativa da literatura feita a partir da seleção de artigos publicados no período de 2012 a 2019. Estudos demonstraram efeitos benéficos da vitamina D3 e seus análogos sobre a função endotelial; no entanto, tais resultados mostram-se controversos, visto que pesquisas com maior amostragem e duração não encontraram redução na morbimortalidade ou nos fatores de risco cardiovascular após o uso de tais substâncias. Frente ao estado atual da arte, não existe embasamento científico claro para suplementação de vitamina D ou seus análogos para tratamento de disfunção endotelial ou doenças cardiovasculares.

Abstract Vitamin D (1,25-dihydroxycolecalciferol) is a prohormone that has attracted the interest of researchers since studies have shown that its effects are not restricted to bone metabolism. Thus, the present review summarizes the most recent findings and discusses the usefulness of prescribing vitamin D and its analogues for treatment and prevention of cardiovascular disorders and endothelial dysfunction. The paper constitutes a narrative review of the literature, selecting articles published from 2012 to 2019. Studies have shown that vitamin D3 and its analogues have beneficial effects on endothelial function, but these results are controversial, since research with larger samples and of longer duration found no reduction in morbidity and mortality or cardiovascular risk factors after use of these substances. Given the current state of the art, there is no clear scientific basis for supplementation with vitamin D or its analogues for treatment of endothelial dysfunction or cardiovascular disease.

ABSTRACT Propolis, is a bee product collected from exudates and flower buds of several plants, has strong aroma and several biological applications. This study aimed at evaluating the chemical composition and in vitro antioxidant, antibacterial and cytotoxic properties of volatile oil from Brazilian brown propolis. It was extracted by hydrodistillation and analyzed by gas chromatography-flame ionization detection and gas chromatography-mass spectrometry. Volatile oil from brown propolis exhibited strong antibacterial activity against H. pylori (MIC 3.25 µg/ml), Mycobacterium tuberculosis (MIC 50 µg/ml) and M. avium (MIC 62.5 µg/ml). It was evaluated in vitro for antioxidant activity by DPPH (IC50 25.0 µg/ml) and ABTS (IC50 30.1 µg/ml) methods. Its cytotoxic property was evaluated in normal (human fibroblasts, GM07429A) and tumor (MCF-7-human breast adenocarcinoma; HeLa-human cervical adenocarcinoma and M059J-human glioblastoma) cell lines. IC50 values were 81.32 µg/ml for GM07429A and 85.00, 129.40 and 84.12 µg/ml for MCF-7, HeLa and M059J cells, respectively. Three major dereplicated components of volatile oil from brown propolis were acetophenone (15.2%), nerolidol (13.3%), and spathulenol (11.6%). Our results contribute to a better understanding of the chemical and biological properties of Brazilian brown propolis and provide evidence for its potential medicinal use.

A Gestão de categorias (GC) é uma ferramenta importante para se fortalecer o relacionamento entre fabricantes e varejistas. Esse processo tem sido associado a grandes varejistas corporativos; contudo, algumas pesquisas recentes mostram que o GC está aberto a empresas de qualquer tipo ou tamanho. Esta possibilidade é importante para mercados emergentes, onde os mercados de bairro ainda são representativos, sendo frequentemente considerados como uma alternativa, para que os fabricantes atinjam margens mais altas do que aquelas alcançadas pelas redes de supermercados maiores. Nesse contexto, o objetivo desta pesquisa foi o de analisar os resultados da iniciativa do de GC, em minimercados de bairro, de uma perspectiva manufatureira. Os dados usados para a elaboração do estudo vêm de uma fabricante de alimentos no Brasil, que implantou um processo de GC junto a 180 pequenos varejistas. Foi utilizada uma análise quantitativa, com o fim de se analisar o efeito do programa nas vendas e na participação de mercado, por parte da fabricante de alimentos. Nossa análise sugere um efeito final positivo do programa, tanto em termos de vendas quanto sobre a participação de mercado.

Category management (CM) is an important tool to strengthen the relationship between manufacturers and retailers. This process has been associated with large corporate retailers; however, some recent researches show that CM is open to companies of any type or size. This possibility is important in emerging markets, where neighborhood supermarkets are still representative and are often considered an alternative for manufacturers to achieve higher margins compared to big chains. In this context, the aim of this research was to analyze the results of a CM initiative in small neighborhood supermarkets from a manufacturer perspective. Data for the study comes from a food manufacturer in Brazil that implemented a CM process with 180 small retailers. A quantitative analysis was conducted in order to analyze the effect of the program on the food manufacturer' s sales and market share. Our analysis suggests an overall positive effect of the program on both, sales and market share.

La Gestión de categorías (GC) es una herramienta importante para fortalecer la relación entre fabricantes y minoristas. Este proceso ha sido asociado a grandes minoristas corporativos; a pesar de eso, algunos datos recientes muestran que el GC está abierto a empresas de cualquier tipo o tamaño. Esta posibilidad es importante para mercados emergentes, donde los mercados de barrio todavía son representativos, siendo frecuentemente considerados como una alternativa, para que los fabricantes alcancen márgenes más altos que en aquellas alcanzadas en las redes de supermercados mayores. En ese contexto, el objetivo de esta investigación fue la de analizar los resultados de la iniciativa del GC, en minimercados de barrio, desde una perspectiva manufacturera. Los datos usados para la elaboración del estudio vienen de un fabricante de alimentos en Brasil, que implantó un proceso de GC junto a 180 pequeños comerciantes. Se utilizó un análisis cuantitativo, con el fin de analizar el efecto del programa en las ventas y en la participación de mercado, por parte del fabricante de alimentos. En nuestro análisis se sugiere un efecto final positivo del programa, tanto en términos de ventas cuanto sobre la participación de mercado.

This study investigated the importance of silicon (Si) in rice grain husk in association with fungicide treatment for preventing Bipolaris oryzae transmission from seed to seedling and the improvement in seedling emergence. Plants from cv. Oochikara and its mutant (lsi1 mutant) defective in active Si uptake were grown in nutrient solution without (-Si) or with (+Si) Si and their panicles were inoculated with B. oryzae. Seeds were evaluated for brown spot severity (BSS) and husk Si concentration. The Si concentration in the husks of cv. Oochikara plants was up to four times higher than the lsi1 mutant plants. BSS was significantly reduced in the husks of the seeds obtained from +Si plants, particularly for cv. Oochikara. A higher percentage of seedling emergence occurred for +Si plant seeds, particularly for cv. Oochikara, and a lower percentage of infected seedlings was observed for the +Si cv. Oochikara treatment, particularly for the fungicide treated seeds. Fungicide was not efficient in preventing seedling infection by B. oryzae for seeds with BSS greater than 50%. High Si concentration in the husk resulted in lower BSS and the fungicide efficiency was greater with a low BSS on seeds from +Si plants, particularly for cv. Oochikara.